Unlock instant, AI-driven research and patent intelligence for your innovation.

a h 2 o 2 Kit for detection of sialic acid with coupled indicator system

A technology of H2O2 and sialic acid, applied in the determination/inspection of microorganisms, measuring devices, instruments, etc., can solve the problems of complicated operation, high instrument cost, poor stability of NADH, etc., and achieve improved accuracy and precision, and obvious color development , the effect of improving the sensitivity and specificity of detection

Active Publication Date: 2020-12-29
王贤俊
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The thiobarbituric acid method, the R-reagent method, and the resorcinol-periodic acid / hydrochloric acid method are complicated to operate and are not suitable for automatic analysis. The equipment costs of the HPLC method and thin-layer chromatography method are relatively high. NAD + - The NADH indicator system measures the concentration or activity of the substance, but NADH has poor stability. There are many dehydrogenases with NADH as the coenzyme in the plasma. It is easy to produce negative reactions with NADH, and there is interference from endogenous pyruvate. Therefore, a A method for detecting sialic acid with high sensitivity and good stability is an urgent technical problem in this field

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • a h <sub>2</sub> o <sub>2</sub> Kit for detection of sialic acid with coupled indicator system
  • a h <sub>2</sub> o <sub>2</sub> Kit for detection of sialic acid with coupled indicator system
  • a h <sub>2</sub> o <sub>2</sub> Kit for detection of sialic acid with coupled indicator system

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0030] Specific Example 1 The kit of the present invention includes the liquid double reagent components of reagent 1 and reagent 2, wherein

[0031]

[0032]

specific Embodiment 2

[0033] Specific embodiment 2 The kit of the present invention comprises the liquid dual reagent components of reagent 1 and reagent 2, wherein

[0034]

[0035]

[0036]

specific Embodiment 3

[0037] Specific embodiment 3 The kit of the present invention includes the liquid dual reagent components of reagent 1 and reagent 2, wherein

[0038]

[0039]

[0040] Specific embodiment 4 precision is measured: with the test kit of measuring sialic acid that embodiment 1 makes, the test kit of measuring sialic acid that embodiment 2 makes, the test kit of measuring sialic acid that embodiment 3 makes The same sample was continuously drawn 20 times for measurement, and the mean, standard deviation and coefficient of variation of the measured values ​​were calculated.

[0041]

[0042] Table 1 Precision test results

[0043]

[0044]

[0045] The coefficient of variation CV is usually used to measure the precision of a determination method, the smaller the CV value, the better the precision of the result of the determination method. For clinical chemistry tests, a method precision with a CV of less than 5% is generally accepted as acceptable. The CV values ​​...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for detecting sialic acid through an H2O2 coupling indicating system. The kit comprises a liquid double-reagent component comprising a reagent 1 and a reagent 2, wherein the volume ratio of the reagent 1 to the reagent 2 is 3 to 1; the reagent 1 comprises a Tris-HCl buffer solution, neuraminidase, ketoamine oxidase, potassium ferrocyanide, tetrahydroxy sodium benzoate and sodium azide; the reagent 2 comprises a Tris-HCl buffer solution, neuraminate aldolase, peroxidase, EDTA, 4-ampyrone, phenol and sodium azide. The kit has the advantages of high sensitivity an good stability, and is worth of further promotion and use.

Description

technical field [0001] The invention relates to the technical field of detection of sialic acid, especially a H 2 o 2 A kit for the detection of sialic acid with a coupled indicator system. Background technique [0002] Currently, sialic acid (Sialic acid, SA): is a derivative of 9-carbon monosaccharide. Usually in the form of oligosaccharides, glycolipids or glycoproteins. It is an important part of cell membrane glycoprotein, related to many biological functions of organisms, and closely related to cell malignant transformation, cancer metastasis, invasion, loss of contact inhibition, decreased cell adhesion and tumor antigenicity. Determination of serum sialic acid concentration can be used as an auxiliary indicator for cancer diagnosis and an indicator for curative effect observation. [0003] Diseases with significantly increased sialic acid include: acute leukemia, esophageal cancer, cardia cancer, gastric cancer, colon cancer, liver cancer, lung cancer, and ovaria...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/527C12Q1/34C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/34C12Q1/527C12Q2326/50C12Q2326/96G01N2333/924
Inventor 王贤俊郑蓓蕾
Owner 王贤俊