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Preparation method for beauveria bassiana granule for preventing and controlling grubs and application thereof

A granule, Beauveria bassiana technology, applied in the directions of botanical equipment and methods, application, pesticides, etc., can solve the problems such as the limitation of popularization and application of Beauveria bassiana, the increase of application cost, the reduction of control effect, etc., and the reduction of spores can be achieved. Damage, reduced processing cost, high vitality effect

Inactive Publication Date: 2017-10-20
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in terms of biological control of grubs, Beauveria bassiana is mainly used for prevention and control. Although Beauveria bassiana has a poisonous effect on grubs, is harmless to humans and animals, and has no pollution to the environment, but Beauveria bassiana preparations are easily affected by the external environment and lose their activity. , resulting in reduced control effects and increased application costs
Therefore, the popularization and application of Beauveria bassiana has been greatly restricted.

Method used

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  • Preparation method for beauveria bassiana granule for preventing and controlling grubs and application thereof
  • Preparation method for beauveria bassiana granule for preventing and controlling grubs and application thereof
  • Preparation method for beauveria bassiana granule for preventing and controlling grubs and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 Beauveria bassiana granule preparation

[0025] 1.1 Preparation of spore powder

[0026] (1) Preparation of Sabouraud’s Medium (SADY): Accurately weigh 40g of glucose, 20g of agar, 10g of yeast extract, and 1000ml of water, prepare the required medium according to the same proportion, sterilize it with high pressure steam for 20min, and cool to 50°C , introduce the culture medium into a sterile Petri dish in a sterile operating bench.

[0027] (2) Inoculate Beauveria bassiana on the SADY plate medium, and culture it at 28°C for 7-9 days. When the surface of the medium is covered with a layer of light yellow or yellow spore powder, dry it with absorbent paper or dry Cover with newspaper and take it out after 36h after dehumidification at 35°C.

[0028] 1.2 Bacteria powder mixing configuration

[0029] Accurately weigh 80-100g of Beauveria bassiana spore powder, 680-720g of carrier, 45-60ml of solvent oil, 15-25g of adhesive, 50-65g of s...

Embodiment 2

[0030] The impact of embodiment 2 carrier on the vigor of Beauveria bassiana

[0031] 1 g of each carrier was weighed, placed in a sterilized test tube containing glass beads and 0.1% Tween-80, stirred evenly with a magnetic stirrer, centrifuged for 24 hours after soaking to obtain the supernatant. Take an appropriate amount of spore powder, put it in a sterilized test tube with glass beads, add the above carrier supernatant, vortex and shake to make the concentration 10 8 100ul of the spore suspension per ml was inoculated on the SDAY medium. 3 replicates per treatment. 10 fields of view were randomly selected from each culture slide to calculate the germination rate. Ears with obvious germ tube growth were recorded as germination, and the spore suspension prepared with 0.1% Tween-80 stored in the same batch and treated as the blank control.

[0032] Table 1 Effects of different carriers on the germination of Beauveria bassiana spores

[0033]

[0034] Analysis of vari...

Embodiment 3

[0035] The impact of embodiment 3 carrier on spore storage activity

[0036] Mix the spore powder and the sterilized carriers evenly according to the mass ratio of 5:95, divide them into 1.5ml centrifuge tubes, seal them and store them at 3°C, 15°C and 25°C respectively. , Take out after 3 weeks and 4 weeks, detect the amount of viable spores (number of colonies) contained in the unit mass, 6 repetitions of each carrier, and use pure spores as a control.

[0037] The effects of different carriers on the storage activity of spores were not the same, but the same carrier had the same effect on the storage activity of spores at different temperatures. Compared with the storage of pure spore powder, calcareous bentonite, kaolin, talcum powder and sugar beet powder can delay the inactivation of spores; but attapulgite is not conducive to the storage of spores.

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Abstract

The invention provides a beauveria bassiana granule for preventing and controlling grubs as well as a preparation method and application thereof. The beauveria bassiana granule is mainly composed of 1-20% of beauveria bassiana spore powder, 70-90% of carrier, 1-20% of solvent oil, 0.05-3% of adhesive, 1-2% of synergist, 1-5% of ultraviolet resistant agent, 1-3% of activator and 0.5-2% of disintegrating agent. The effect for preventing and controlling grubs can reach up to 80% or above. Compared with the other agents, the beauveria bassiana granule has the advantages in crop output, rate of insect mortality and application mode. According to the invention, beet powder is used as a carrier, the acting object of the pests is transferred from the plant to the granule preparation, the grubs are lured and killed and the insecticide efficiency of the beauveria bassiana spore powder is increased, so that the usage of the chemical drugs is reduced, the environment is protected and the technical support is supplied for the development of organic agriculture.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to Beauveria bassiana granules for preventing grubs, a preparation method and application thereof. Background technique [0002] The grub (Holotrichia diomphalia H.morosa) is the larva of the Coleoptera beetle, also known as the walnut worm and the white soil silkworm. It has a variety of food habits and a large amount of food, and it lives underground. The grub hazard is hidden and lagging, and it is recognized as a major soil-dwelling pest that is difficult to predict and control at home and abroad. Grubs often cause severe damage to rhizomes, wilting and death of plants, and reduced or even failure of crops. In recent years, with the adjustment of planting structure and the rapid development of large-scale conservation tillage systems, the population density of grubs in many areas has been increasing, resulting in aggravated damage. Due to the biologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N25/12A01N25/08A01N25/22A01N47/34A01N43/16A01N37/44A01P7/04
CPCA01N25/006A01N25/08A01N25/12A01N25/22A01N37/44A01N43/16A01N47/34A01N63/30
Inventor 李娇娇战伟伟邱磊李敬龙
Owner QILU UNIV OF TECH