Tissue culture and rapid propagation method for prune
A technology for tissue culture and apricot plum, applied in the field of plant tissue culture, can solve the problems of few clump stems, slow differentiation of in vitro tissue culture, difficult rooting, etc., and achieves the effects of simple operation, increased transplanting survival rate, and solving pollution problems.
Active Publication Date: 2017-10-24
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology
[0008] Although the above-mentioned organogenesis pathways such as leaf and embryo culture and somatic embryogenesis pathways have obtained regenerated plants, there are still problems such as slow differentiation in vitro tissue culture, few clustered stems, and difficulty in rooting, which cannot meet the actual production needs and plum blossom genetics. conversion action
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Embodiment 1
[0050] Apricot plum tissue culture rapid propagation method is as follows:
[0051] 1. Experimental method
[0052] 1. Seed disinfection treatment: Use the core clamp to break the ripe fruit core of apricot plum and collect the complete seeds. Carry out the following three methods of disinfection respectively:
[0053] (1) Use 75% alcohol to sterilize for 30 seconds, and then transfer to 3% sodium hypochlorite to sterilize for 20 minutes in an ultra-clean workbench;
[0054] (2) After rinsing with running water for 30 minutes, in the ultra-clean workbench, transfer to 2% sodium hypochlorite for disinfection for 30 minutes;
[0055] (3) After rinsing with running water for 30 minutes, disinfect with 2% sodium hypochlorite (containing 0.03% Tween-20) for 30 minutes in an ultra-clean workbench. The seeds sterilized by the three methods are all washed 3-4 times with sterile water;
[0056] 2. Seed cold storage treatment: transfer the sterilized seeds into the cold storage medi...
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The invention relates to a tissue culture and rapid propagation method for prune. The method comprises the steps of adopting annual prune seeds as explants, carrying out disinfection, refrigeration, initiation culture, inoculating cotyledons and / or tender stems of aseptic seedlings into a differential medium and carrying out cultivation for 3-4 weeks; obtaining adventitious buds, transferring the adventitious buds to a stooling medium and carrying out further cultivation for 2-3 weeks; when tufted seedlings grow to 3-4cm, transferring the tufted seedlings into a rooting medium and carrying out cultivation for 2-3 weeks; and transplanting rooted seedlings, hardening the seedlings through a cover and carrying out normal cultivation. The induction quantity of adventitious buds reaches 10.6, the heights of the stems of the tufted seedlings reach 3.56cm, the enhancement factors of the tufted seedlings reach 5.5 and the rooting rate reaches 95%. Tissue culture and rapid propagation of the prune are achieved, and a technical support is provided for genetic transformation and molecular breeding of plum blossom.
Description
technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a rapid propagation method of apricot plum tissue culture. Background technique [0002] Plum blossom (Prunus mume) is one of the ten famous traditional Chinese flowers. It is an important ornamental flower and fruit tree with colorful flowers and abundant branches. The cultivation range of plum blossoms has been limited to the Yangtze River and Huaihe River basins for more than 2,000 years. In order to expand the scope of cultivation of this traditional famous flower, especially to promote the application of plum blossoms to the Sanbei region, cold-resistant plum blossom breeding has been carried out half a century ago. Work. In the early days, through the interspecific hybridization of true plum varieties and apricot and other related species, apricot plum varieties with cold resistance were bred, which enriched the plant landscape in early spring in the north. ...
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IPC IPC(8): A01H4/00A01C1/08
CPCA01C1/08A01H4/001A01H4/008
Inventor 郑唐春张启翔卓孝康孙丽丹袁存权程堂仁王佳
Owner BEIJING FORESTRY UNIVERSITY