Method for establishing meriones meridianus hepatic alveolar echinococcosis animal model

An animal model and construction method technology, applied in the medical field, can solve the problems such as the inability to obtain a breakthrough in the construction technology of the animal model of alveolar sacculus disease of Meridian gerbil, and achieve wide practicability, low infection rate and good reproducibility. Effect

Active Publication Date: 2017-10-27
THE CENT FOR DISEASE CONTROL & PREVENTION OF XINJIANG UYGUR AUTONOMOUS REGION
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Problems solved by technology

In the prior art, there are very few reports on the animal model of pulmonary echinococcosis. There is only one document that the applicant applied to the National Natural Science Foundation of China when studying the habits of Meridian gerbils. Establishment of Animal Model" project, the content recorded in the project is only the applicant's initial research on Meridian gerbil, which laid the foundation for how to establish an animal model of pulmonary echinococcosis, but how to successfully establish an animal model of Meridian gerbil alveolar coccidiosis The model still needs further research, and it is not possible to obtain a breakthrough in the construction of an animal model of meridian gerbil alveolar coccidiosis

Method used

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  • Method for establishing meriones meridianus hepatic alveolar echinococcosis animal model
  • Method for establishing meriones meridianus hepatic alveolar echinococcosis animal model
  • Method for establishing meriones meridianus hepatic alveolar echinococcosis animal model

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Experimental program
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Effect test

Embodiment 1

[0039] The specific steps of constructing the alveolar coccidiosis animal model of Meridian gerbil are as follows:

[0040] (1) Experimental animals: Select 40 clean-grade closed group Meridian gerbils, 8-12 weeks old, half male and half male, and 40 clean-grade BALB / c mice, 8-10 week old, half male and half male.

[0041] (2) Breeding of Meridian gerbils: conventional feeding mode, feed type: cobalt 60 irradiated rats maintained feed, ad libitum, 10 g of carrots and 5 g of sunflower seeds per week were added, and the drinking water was subjected to high pressure Sterilization.

[0042] (3) Cyst removal: Echinococcus multilocularis subcultured BALB / c mice were sacrificed under carbon dioxide anesthesia, and Echinococcus multilocularis cysts were removed by aseptic surgery.

[0043] (4) Grinding and washing the cysts: cut the cysts into pieces and grind them in a mortar, pass through an 80-mesh sieve after fully grinding, and wash the sieves with the double antibody solution a...

Embodiment 2

[0055] Select 40 clean-grade closed group Meridian gerbils, 8-12 weeks old, half male and half male, and 40 clean-grade BALB / c mice, 8-10 week old, half male and half male, all adopt the conventional feeding mode, and eat freely Rats irradiated with cobalt 60 were kept in feed, with carrots 10g / rat and sunflower seeds 5g / rat added every week, and the drinking water was sterilized by autoclaving. Echinococcus multilocularis subcultured BALB / c mice were sacrificed by carbon dioxide anesthesia, and Echinococcus multilocularis cysts were dissected by aseptic surgery. Rinse the screen with a double-antibody solution prepared by dissolving 500,000 units of penicillin and 500,000 units of streptomycin in 500ml of 0.9% sterile saline, inhale the filtrate with a 50ml syringe, add the double-antibody solution to precipitate the protoscolmus, and discharge the supernatant , repeated three times, mixed the original scorch suspension, stained with 0.5% eosin, dropped into a hemocytometer f...

Embodiment 3

[0058] Select 40 clean-grade closed group Meridian gerbils, 8-12 weeks old, half male and half male, and 40 clean-grade BALB / c mice, 8-10 week old, half male and half male, all adopt the conventional feeding mode, and eat freely Rats irradiated with cobalt 60 were kept in feed, with carrots 10g / rat and sunflower seeds 5g / rat added every week, and the drinking water was sterilized by autoclaving. Echinococcus multilocularis subcultured BALB / c mice were sacrificed by carbon dioxide anesthesia, and Echinococcus multilocularis cysts were dissected by aseptic surgery. Rinse the screen with a double-antibody solution prepared by dissolving 500,000 units of penicillin and 500,000 units of streptomycin in 500ml of 0.9% sterile saline, inhale the filtrate with a 50ml syringe, add the double-antibody solution to precipitate the protoscolmus, and discharge the supernatant , repeated three times, mixed the original scorch suspension, stained with 0.5% eosin, dropped into a hemocytometer f...

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Abstract

The invention discloses a method for establishing a meriones meridianus hepatic alveolar echinococcosis animal model. By adopting a series of technical means, such as screening of experimental animals, dissection and washing of cysts, preparation of protoscoleces suspension, microscopic examination of protoscoleces, anesthesia of meriones meridianus and modeling, the method for establishing the meriones meridianus hepatic alveolar echinococcosis animal model is creatively provided. According to the hepatic alveolar echinococcosis animal model established by means of the method, 60 d cyst tissue almost covers the whole thoracic cavity and completely covers the lung, and 15d, 45d and 60d meriones meridianus cyst coefficient is significantly different from the BALB / c mice cyst coefficient. The provided method for establishing the meriones meridianus hepatic alveolar echinococcosis animal model has the advantages of being high in molding rate, short in molding cycle, low in cost and good in repeatability, and the method in widely used in the medical technical field and also practical for the medical technical field.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular, the invention relates to the technical field of a method for establishing an animal model of alveolar coccidiosis. Background technique [0002] Echinococcosis is a zoonotic parasitic disease that occurs mostly in pastoral areas, and is mainly caused by the larvae of Echinococcus granulosus (Echinococcus granulosus.Eg) and larvae of Echinococcus multilocularis (Echinococcus multilocularis.Em). Among them, the alveolar echinococcosis caused by the larvae of Echinococcus multilocularis (alveolar echinococcosis) is also called alveolar echinococcosis (Ae) because of the infiltrative growth characteristics of the cysts, which makes the disease extremely difficult to cure. Known as "worm cancer". In areas with high echinococcosis prevalence, echinococcosis has become a public health problem that restricts local economic development and affects social stability. More than 15% of human...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02A01K2207/30A01K2227/105A01K2267/035A01K2267/0393
Inventor 史深徐艺玫罗芸燕顺生徐晓辉袁江玲闫化奎补娟
Owner THE CENT FOR DISEASE CONTROL & PREVENTION OF XINJIANG UYGUR AUTONOMOUS REGION
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