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Method for separating and purifying eicosapentaenoate and docosahexaenoate by simulated moving bed chromatography

A technology for simulating moving bed and carbapentaenoic acid esters, which is applied in chemical instruments and methods, preparation of carboxylic acid esters, preparation of organic compounds, etc., can solve the problems of complicated SMB separation and purification process, increased equipment cost and solvent consumption, etc. Achieve the effect of saving equipment consumption, saving solvent and reducing cost

Active Publication Date: 2020-12-29
NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional simulated moving bed can only divide a mixture into two fractions at a time, so a total of 4 simulated moving bed operations are required to produce EPA ester and DHA ester from ester-type fish oil.
For example, one SMB operation divides ester-type fish oil into two fractions: Fraction 1 contains EPA esters and pre-impurities, and fraction 2 contains intermediate impurities, DHA esters, and post-impurities; and then separates EPA esters and DHA ester, which undoubtedly makes the SMB separation and purification process more complicated, and also increases equipment costs and solvent consumption, it is necessary to develop a more practical and reasonable separation process

Method used

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  • Method for separating and purifying eicosapentaenoate and docosahexaenoate by simulated moving bed chromatography
  • Method for separating and purifying eicosapentaenoate and docosahexaenoate by simulated moving bed chromatography
  • Method for separating and purifying eicosapentaenoate and docosahexaenoate by simulated moving bed chromatography

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0044] Embodiment 1 (see figure 1 shown)

[0045] 2 pillars are arranged in Zone I, Zone II and Zone III respectively. and disable as image 3 The circulating pump P4 in zone IV of the traditional simulated moving bed equipment shown (equivalent to not using zone IV, only an open-loop simulated moving bed with three zones).

[0046] Step 1—removal of pre-impurity and post-impurity feed: the raw material liquid and solution 2 are pumped into the simulated moving bed separation system from the feed port 3 and the eluent inlet 1 at the flow rate of 1mL / min and 4mL / min respectively; II Zone flow control is 3mL / min. Every 5 minutes, the eluent inlet 1 and feed inlet 3 are switched to the next column inlet along the liquid flow direction, while the raffinate outlet 4 and extract outlet 2 flow along the liquid flow direction. Direction moves to the next column exit. The composition of the raffinate and the extract was analyzed by high performance liquid chromatography, and the r...

Embodiment 2

[0050] Embodiment 2 (see figure 2 shown)

[0051] Adopt the simulated moving bed among the embodiment 1, but disconnect the I zone and II zone wherein, solution 2 directly flows into II zone through II zone inlet 5, and wash I zone with pure methanol, and I zone effluent liquid is all collected for the extract. All the other operating conditions and operating steps are the same as in Example 1. The experimental results show that the product separation purity does not change.

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Abstract

The invention discloses a method of separating and purifying eicosapentaenoate and docosahexaenoate via simulated moving bed chromatography. The separation step includes: enabling eluting capacity of a liquid phase in region II to be higher than that of a liquid phase in region III so that EPA (eicosapentaenoic acid) ester, DHA (docosahexaenoic acid) ester and intermediate impurity are intercepted in the region II and the region III, with pre-impurity moving forward with the liquid phases and flowing out of a raffinate outlet, and post-impurity backing with column switching and flowing out of the raffinate outlet; adjusting flow quantities and compositions of liquid phases flowing in the region II and region III or adjusting switching time so that the intermediate impurity is intercepted in the system, with the EPA ester moving forward with the liquid phases and flowing out of the raffinate outlet, and DHA ester backing with column switching and flowing out of the raffinate outlet; flushing out target components intercepted in a simulated moving bed for the purpose of recycling. The simulated moving bed chromatography system of the invention allows EPA ester and DHA ester to be separated from disposable bis-ester fish oils, thereby saving energy and reducing production cost.

Description

technical field [0001] The invention relates to a method for separating and purifying eicosapentaenoic acid (EPA) ester and docosahexaenoic acid (DHA) ester by using simulated moving bed chromatography. Background technique [0002] Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are two important active substances present in deep sea fish oil, which have various physiological effects on human health. For example, EPA can inhibit the formation of thrombus, lower triglycerides, thereby avoiding the occurrence of cardiovascular and cerebrovascular diseases; DHA can promote the re-extension of brain cell protrusions, improve the information transmission ability between brain cells, so it can promote the development of infant brain, It can also prevent senile dementia. [0003] EPA and DHA in fish oil are mainly in the form of glycerides. Generally, the fish oil is methylated or ethylated first, and the glycerides of EPA and DHA are converted into methyl or ethyl est...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/587C07C67/56
CPCC07C67/56C07C69/587
Inventor 危凤金春华
Owner NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG