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Extraction method of malonyl ginsenoside and application of malonyl ginsenoside in treatment of diabetic nephropathy

A technology of malonyl ginsenosides and ginsenosides is applied in the application of preparing prevention and treatment of diabetic nephropathy, and the extraction field of malonyl ginsenosides can solve the difficulty of separation and purification, the low extraction rate of the preparation process, and the extraction of malonyl ginsenosides. To solve the problem of low rate, to achieve the effect of low-cost enrichment, improved purity and yield

Active Publication Date: 2017-11-17
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention discloses a method for extracting and purifying malonyl ginsenoside, which solves the disadvantages of low extraction rate, difficult separation and purification and low yield of high-purity malonyl ginsenoside in the existing preparation process.

Method used

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  • Extraction method of malonyl ginsenoside and application of malonyl ginsenoside in treatment of diabetic nephropathy
  • Extraction method of malonyl ginsenoside and application of malonyl ginsenoside in treatment of diabetic nephropathy
  • Extraction method of malonyl ginsenoside and application of malonyl ginsenoside in treatment of diabetic nephropathy

Examples

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Embodiment 1

[0033] Fresh ginseng (fibrous root, branch root, rhizome, tap root, etc.) was used as raw material, chopped, and 2 times the amount of distilled water was added; then cellulase with 1% of the mass of the ginseng sample was added, and the water bath was shaken at a constant temperature of 20°C for 2 hours. After enzymatic hydrolysis, an appropriate amount of distilled water was added to make the amount of the solvent 10 times, and then ultrasonically extracted for 3 times, each time for 0.5 h. Recover the solvent under reduced pressure at 40°C, and concentrate it into an aqueous solution; then use NKA-12 adsorption resin for separation, and the eluent is ethanol and 0.01mol / L K 2 HPO 4 Gradient elution, collect the eluted fractions separately, detect by thin-layer chromatography, collect and combine the fraction solutions with the same Rf value as the positive reference substance of malonyl ginsenoside, recover the solvent under reduced pressure, and use dextran as the concentr...

Embodiment 2

[0035] Fresh ginseng fibrous roots, branch roots and main roots were used as raw materials, chopped, and three times the amount of distilled water was added; then cellulase with 5% mass of the ginseng sample was added, and the water bath was shaken at a constant temperature of 40°C for 4 hours. After enzymatic hydrolysis, appropriate amount of ethanol and water were added so that the concentration of ethanol was 20%, and the amount of solvent used was 30 times, and then ultrasonically extracted 4 times, 1 hour each time. Recover the solvent under reduced pressure at 40°C, concentrate the extract into an aqueous solution, and then use NKA-12 adsorption resin for separation. The eluent is ethanol and 0.02mol / L KH 2 PO 4 Gradient elution, collect the eluted fractions separately, detect by thin-layer chromatography, collect and combine the fraction solutions with the same Rf value as the positive reference substance of malonyl ginsenoside, recover the solvent under reduced pressur...

Embodiment 3

[0037] Fresh American ginseng was used as raw material, chopped, and 5 times the amount of distilled water was added; then cellulase with 10% of the sample mass of American ginseng was added, and the water bath was shaken at a constant temperature of 60°C for 6 hours. After enzymatic hydrolysis, appropriate amount of ethanol and water were added so that the concentration of ethanol was 40%, and the amount of solvent used was 50 times, and then ultrasonically extracted 5 times, each time for 2 hours. Recover the solvent under reduced pressure at 40°C and concentrate the extract into an aqueous solution; then use NKA-12 adsorption resin for separation, eluent is ethanol and 0.05mol / L Na 2 HPO 4 Gradient elution, collect the eluted fractions separately, detect by thin-layer chromatography, collect and combine the fraction solutions with the same Rf value as the positive reference substance of malonyl ginsenoside, recover the solvent under reduced pressure, and use dextran as the ...

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Abstract

The invention provides an extraction method of malonyl ginsenoside and application of the malonyl ginsenoside in treatment of diabetic nephropathy. The extraction method comprises the following steps: by taking fresh ginseng or American ginseng as a raw material, carrying out cellulase enzymatic hydrolysis treatment, then carrying out ultrasonic extraction, carrying out reduced pressure solvent recycling, concentrating into aqueous solution, then separating with NKA-12 adsorption resin, carrying out gradient elution with ethanol and phosphate buffer salt solution, and desalting with sephadex gel, so that the malonyl ginsenoside is obtained. The extraction method efficiently utilizes cellulase for degrading cell walls of ginseng and American ginseng, so that extraction ratio of the malonyl ginsenoside is improved, and the extraction method has the characteristics of simple operation, rapidness, low cost and high extraction efficiency; and animal experiments prove that the malonyl ginsenoside has obvious prevention and treatment effects on type 2 diabetic nephropathy and can be applied to preparation of health food or medicines.

Description

technical field [0001] The present invention relates to the preparation method of Chinese herbal extracts and its application in the preparation of medicines, especially discloses a method for extracting malonyl ginsenosides, and also provides its application in the preparation of prevention and treatment of diabetic nephropathy, which belongs to traditional Chinese medicine technology field. Background technique [0002] Diabetic nephropathy (diabetic nephropathy, DN) is one of the most serious complications of diabetes, and it is also one of the main causes of clinical death in diabetic patients. According to reports, about 20% to 40% of patients with type Ⅰ or type Ⅱ diabetes will develop nephropathy; in my country, the incidence of diabetic nephropathy is about 40%. The occurrence of diabetic nephropathy is the result of multiple factors: glucose metabolism disorders, changes in renal hemodynamics, various cytokines and genetic background all play a very important role....

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P3/10A61P13/12
CPCA61K36/258A61K2236/19A61K2236/333A61K2236/39A61K2236/55
Inventor 刘志孙光芝阮长春
Owner JILIN AGRICULTURAL UNIV
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