Algicide and preparation method thereof
A technology of algaecide and organic extractant, applied in herbicides and algicides, botanical equipment and methods, biocides, etc., can solve problems such as unsuitable fish and vegetable coculture systems, chemical pollution of algaecides, etc. To achieve the effect of improving the inhibitory effect, prolonging the action time, and enhancing the inhibitory effect
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Embodiment 1
[0027] A preparation method of algicide, comprising the following steps:
[0028] (1) Configure YPD medium, add 4% chitin powder and 5% sodium chloride by weight of the medium, and then inoculate 0.5% yeast by weight of the medium, culture at 25°C for 48 hours, and extract Yeast extracellular substance, spare;
[0029] (2) Crush the root of water hyacinth, add organic extractant, extract 2~3 times, 3 hours each time, then combine the extracts, concentrate under reduced pressure, evaporate the organic solvent to obtain the extract; wherein, organic extractant and water hyacinth The volume-to-weight ratio of the powder is 6:1; the organic extractant includes acetone and ethyl acetate, and the volume ratio of the two is 5:0.8;
[0030] (3) Mix the yeast extracellular substance and the extract, and add copper sulfate and 5% calcium chloride accounting for 10% by weight of the yeast extracellular substance, stir evenly, then add it to polycaprolactone, and It is prepared as a gra...
Embodiment 2
[0032] A preparation method of algicide, comprising the following steps:
[0033] (1) Configure YPD medium, add 2.4% chitin powder and 3.3% sodium chloride by weight of the medium, and then inoculate 0.5% yeast by weight of the medium, culture at 25°C for 40 hours, and extract Yeast extracellular substance, spare;
[0034] (2) Crush the root of water hyacinth, add organic extractant, extract 2~3 times, 3 hours each time, then combine the extracts, concentrate under reduced pressure, evaporate the organic solvent to obtain the extract; wherein, organic extractant and water hyacinth The volume-to-weight ratio of the powder is 5:1; the organic extractant includes acetone and ethyl acetate, and the volume ratio of the two is 3:0.8;
[0035] (3) Mix the yeast extracellular substance and the extract, add 5.6% copper sulfate and 4.2% calcium chloride accounting for the weight of the yeast extracellular substance, stir evenly, then add it to the polycaprolactone, and It is prepared ...
Embodiment 3
[0037] A preparation method of algicide, comprising the following steps:
[0038] (1) Configure YPD medium, add 2% chitin powder and 3% sodium chloride by weight of the medium, and then inoculate 0.5% yeast by weight of the medium, culture at 25°C for 42 hours, and extract Yeast extracellular substance, spare;
[0039](2) Crush the root of water hyacinth, add organic extractant, extract 3 times, each time for 2 hours, then combine the extracts, concentrate under reduced pressure, evaporate the organic solvent to obtain the extract; wherein, the organic extractant and water hyacinth powder The volume-to-weight ratio is 6:1; the organic extractant includes acetone and ethyl acetate, and the volume ratio of the two is 3:1;
[0040] (3) Mix the yeast extracellular substance and the extract, and add copper sulfate and 5% calcium chloride accounting for 10% by weight of the yeast extracellular substance, stir evenly, then add it to polycaprolactone, and It is prepared as a granula...
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