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Liquid chromatography systems, devices and methods

A technology of liquid chromatography, virus inactivation, applied in the field of devices for processing said biomolecules and/or fluids

Active Publication Date: 2020-11-03
CYTIVA SWEDEN AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A continuous process involving viral inactivation and other modification of biomolecules and / or fluids presents challenges in suitable equipment and methods

Method used

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  • Liquid chromatography systems, devices and methods
  • Liquid chromatography systems, devices and methods
  • Liquid chromatography systems, devices and methods

Examples

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example 1

[0045] Example 1 – Serial virus inactivation in Mab treatment using two containers (loading loop)

[0046] Feeds consisting of monoclonal antibody (Mab) fluids were received from a continuous cell culture process and captured by affinity chromatography using protein A media (MabSelect®, available online from GE Healthcare) according to well known techniques.

[0047] Virus inactivation is included in the continuous process according to the invention by providing two containers in parallel (also called loading loop) as described herein. Each sample loop is equipped with a bi-directional movable seal that allows fluid to be applied and dispensed from the top and bottom of the loop. A pH-adjusted fluid Mab feed generated by the chromatography column was applied to the first loop (Loop 1 ). When ring 1 was filled to a satisfactory level with fluid, it remained in the vessel for 30 minutes while the Mab feed from the column was switched and applied to the second ring (ring 2). Wh...

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Abstract

The present invention relates to a liquid chromatography system for the separation of biomolecules in a fluid comprising at least two unit operations, wherein the first unit operation is a step of multi-column chromatography and the second unit operation is the step of analyzing said biomolecules and / or or the step of fluid modification, wherein the modification comprises feeding the fluid produced by the last chromatographic column of the first unit operation into a system comprising at least two vessels, wherein each vessel has a space and is arranged to divide the space into a first movable side walls of the subspace and the second subspace, and each container includes a first port connected to the first space and a second port connected to the second subspace. The invention also relates to a virus inactivation device for a chromatography system capable of continuous or semi-continuous processing of biomolecules according to the invention and a method of using such a device.

Description

technical field [0001] The present invention relates to liquid chromatography, and more particularly to systems for separating biomolecules in fluids, devices capable of processing said biomolecules and / or fluids and methods of using the devices according to the invention. Background technique [0002] Liquid chromatography is one of the most commonly used separation principles in the analysis and manufacture of biomolecules such as proteins and peptides. There are many commercial instruments available today for processing biomolecules by chromatography, most of which are capable of automated processing. However, most liquid chromatography systems require some human intervention, such as sample application, changes in operating parameters, etc., which can make their use time-consuming and more laborious than desired. [0003] To speed up the processing of biomolecules, where possible, improvements are also being made towards continuous processing. One method that is increa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/60G01N30/16
CPCG01N30/16G01N30/6034B01D15/14G01N30/465G01N2030/208G01N2030/8813B01D15/1864B01D15/1807B01D15/3809C07K1/22C12N7/00
Inventor B.M.奥洛夫斯森A.荣格洛夫K.M.拉基
Owner CYTIVA SWEDEN AB