Antigen or antibody coated magnetic particle method, application and kit
A technology of magnetic particles and antigens, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems affecting the calibration cycle and validity period of the kit, poor stability, etc., and achieve the effect of good stability and high detection signal value
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Embodiment 1
[0065] The one-step method, the two-step method and the method of the present invention are used to coat the RNP antigen on the magnetic particles, matched with the HRP-anti-human IgG antibody, and respectively detect the main calibrator CAL1-CAL6 of the RNP project. Antigen magnetic particles with higher detection signal value were placed in a 37°C water bath for 7 days and 14 days, and then matched with HRP-anti-human IgG antibody to detect the main calibrator CAL1-CAL6 of the RNP project respectively. After 7 days and 14 days of water bathing at 37°C, the signal retention rate of the antigen magnetic particles was calculated.
[0066] The preparation method of the master calibrator of the RNP project is as follows:
[0067] CAL1: Sample Diluent No. 4
[0068] CAL2: Sample Diluent No. 4 dilutes the Anti-RNP Control high value point by 4096 times
[0069] CAL3: Sample Diluent No. 4 dilutes the Anti-RNP Control high value point by 512 times
[0070] CAL4: Sample Diluent No....
Embodiment 2
[0097] Using one-step method, two-step method and the above-mentioned optimization process, the SCL-70 antigen is coated with magnetic particles, matched with HRP-anti-human IgG antibody, and the main calibrator CAL1-CAL6 of the SCL-70 project is detected. Place the antigen magnetic particles with higher detection signal value in a 37°C water bath for 7 days and 14 days, and then match with HRP-anti-human IgG antibody to detect the main calibrator CAL1-CAL6 of the SCL-70 project. After 7 days and 14 days of water bathing at 37°C, the signal retention rate of the antigen magnetic particles was calculated.
[0098] The preparation method of antigen-coated magnetic particles is as follows:
[0099] The preparation method of the main calibrator of the SCL-70 project is as follows:
[0100] CAL1: Sample Diluent No. 4
[0101] CAL2: Sample Diluent No. 4 dilutes Anti-Scl-70 Control high value point by 4096 times
[0102] CAL3: Dilute the Anti-Scl-70 Control high value point by 512...
Embodiment 3
[0129] The anti-HBeAg monoclonal antibody-1 was coated with magnetic particles and matched with HRP-HBeAb monoclonal antibody to detect recombinant hepatitis B virus e antigen main calibrator CAL1-CAL7 by one-step method, two-step method and the above-mentioned optimized process. The magnetic particles with higher detection signal value were placed in a 37-degree water bath for 7 days and 14 days, and then matched with HRP-HBeAb monoclonal antibody to detect recombinant hepatitis B virus e antigen main calibrator CAL1-CAL7. Calculate the retention rate of antibody magnetic particle signal after 7 days and 14 days in 37 degree water bath.
[0130] The preparation method of the recombinant hepatitis B virus e antigen master calibrator is as follows:
[0131] CAL1: Sample Diluent No. 4
[0132] CAL2: Sample diluent No. 4 dilutes recombinant hepatitis B virus e antigen 30,000 times
[0133] CAL3: Sample diluent No. 4 dilutes recombinant hepatitis B virus e antigen 8000 times
...
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