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Microbial compound bacterial agent for deodorization

A compound bacterial agent and microorganism technology, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of waste of manpower and material resources, unsustainable deodorization effect, etc., achieve low production cost, prevent and deodorize Source, effect of eliminating odor source substances

Inactive Publication Date: 2017-12-19
WUXI SHENGYA BIOTECH CO LTD FOSHAN BRANCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The use of microbial agents can remove the source of odor on the surface of garbage or domestic sewage to a certain extent, but there are also some problems in the use of microbial treatment. Deodorization has caused a lot of waste of manpower and material resources. Therefore, it is necessary to provide a microbial composite bacterial agent with a long deodorization duration to meet the requirements of environmental protection.

Method used

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  • Microbial compound bacterial agent for deodorization

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preparation example Construction

[0028] 1. Preparation of microbial compound bacterial agent

[0029] 1. Embodiment 1

[0030] A microbial composite bacterial agent can be prepared by the following method:

[0031] A: Preparation of fermentation broth of yeast strains: activate Kluyveromyces marx and inoculate them in the liquid medium for cultivating yeasts, cultivate them in a shaker at 120r / min for 72 hours at 28°C, count them and use sterile water to adjust the concentration of K. marx to 1 x 10 7 CFU / mL.

[0032] B: Preparation of EM strain fermentation broth: add 0.2Kg brown sugar to warm water, stir and dissolve thoroughly, add 10g EM strain when cooled to room temperature, and seal and ferment at 25°C for 5 days to obtain EM fermentation broth.

[0033] C: Mix the fermented liquid of the yeast strain and the fermented liquid of the EM strain uniformly at a volume ratio of 0.2:1 to obtain the microbial composite bacterial agent of the present invention.

Embodiment 2

[0035] A microbial composite bacterial agent can be prepared by the following method:

[0036] A: Preparation of yeast strain fermentation broth: activate Kluyveromyces marx and inoculate it in the liquid medium for cultivating yeast, cultivate it in a shaker at 180r / min for 48h at 30°C, count it with a sterile water to adjust the concentration of K. marx to 1 x 10 9 CFU / mL.

[0037] B: Preparation of EM strain fermentation liquid: add 0.4Kg brown sugar to warm water, stir and dissolve thoroughly, add 6 g of EM strain when cooled to room temperature, and seal and ferment at 25°C for 8 days to obtain EM fermentation liquid.

[0038] C: Mix the fermented liquid of the yeast strain and the fermented liquid of the EM strain uniformly at a volume ratio of 0.3:1 to prepare the microbial composite bacterial agent of the present invention.

Embodiment 3

[0040] A microbial composite bacterial agent can be prepared by the following method:

[0041] A: Preparation of fermentation broth of yeast strains: activate Kluyveromyces marx and inoculate them in the liquid medium for cultivating yeasts, cultivate them in a shaker at 150r / min for 60 hours at 36°C, count them and use sterile water to adjust the concentration of K. marx to 1 x 10 8 CFU / mL.

[0042] B: Preparation of EM strain fermentation broth: add 0.3Kg brown sugar to warm water, stir to dissolve, when cooled to room temperature, add 8g EM strain, and seal and ferment at 25°C for 6 days to obtain EM fermentation broth.

[0043] C: Mix the fermented liquid of the yeast strain and the fermented liquid of the EM strain uniformly at a volume ratio of 0.5:1 to obtain the microbial composite bacterial agent of the present invention.

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Abstract

The invention discloses a microbial compound bacterial agent for deodorization and an application. The microbial compound bacterial agent contains kluyveromyces marxianus and EM bacteria and can be prepared by the following steps: fermenting the kluyveromyces marxianus and the EM bacteria respectively; mixing the kluyveromyces marxianus and the EM bacteria in a volume ratio of (0.2-0.5) to 1. According to the microbial compound bacterial agent disclosed by the invention, the preparation method is simple, peculiar smell can be quickly removed, and the source of repugnant substances is eliminated; moreover, the lasting deodorization effect is excellent; after 8 days since spraying, the deodorization effect still can be maintained at a relatively high level.

Description

technical field [0001] The invention belongs to the fields of environmental protection and microorganisms, and in particular relates to a microbial composite bacterial agent for deodorization and a preparation method thereof. Background technique [0002] With the development of industrial and agricultural production and the improvement of people's living standards, the pollutants produced by human beings have been increasing. Soil and water bodies have been polluted to varying degrees, which has seriously affected and damaged the virtuous cycle of the ecological environment, and has also caused harm to human health and self-development. Environmental pollution has increasingly become one of the common issues of various countries. , environmental pollution and protection has become a major issue in today's international community, especially in developing countries, the control of environmental pollution has reached the point of no delay. An important cause of pollution is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16B01D53/84B01D53/58B01D53/52C12R1/645
CPCB01D53/52B01D53/58B01D53/84B01D2257/90C12N1/16C12N1/20Y02A50/20
Inventor 庞婉青
Owner WUXI SHENGYA BIOTECH CO LTD FOSHAN BRANCH
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