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A monitoring method for the production of trehalose by single enzyme method

A technology of trehalose and trehalose synthase is applied in various fluid ratio control, ratio control, chemical variable control and other directions to achieve accurate and reliable results, reduce monitoring and production costs, and save labor.

Active Publication Date: 2020-04-21
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to change the frequent detection of trehalose content manually in the process of single-enzyme production of trehalose into near-infrared automatic detection and automatically control the amount of alkali added according to its changes to achieve the purpose of automatic regulation of production optimization , that is, to provide a method: apply near-infrared real-time detection of trehalose content in the process of single-enzyme production of trehalose, establish a model and correct it by calculating and analyzing the relationship between the change of its content and the change of pH value, and then determine the time and time of adding alkali The amount of alkali is used to stabilize the pH value of the reaction solution so that it is in the optimum pH range with the highest activity of trehalose synthase, so as to achieve the purpose of automatic regulation of production optimization

Method used

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  • A monitoring method for the production of trehalose by single enzyme method
  • A monitoring method for the production of trehalose by single enzyme method
  • A monitoring method for the production of trehalose by single enzyme method

Examples

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Effect test

Embodiment 1

[0015] Embodiment 1 monitoring system is installed: as figure 1 As shown, the near-infrared real-time monitoring and control system is installed, including reaction tank (1), throttle valve (2), detection pool (3), optical fiber (4), near-infrared spectrometer (5), computer (6), plus Alkaline pool (7) and throttle valve (8), the throttle valve (2) is set at the outlet of the reaction tank (1), the detection pool (3) is connected to the optical fiber (4) on both sides, the optical fiber (4) and the near-infrared spectrometer (5) connection, the near-infrared spectrometer (5) is connected with the computer (6), and the computer is connected with the alkali-adding pool (7) simultaneously, and the alkali-adding pool (7) is connected with the reaction tank (1) through the throttle valve (8).

Embodiment 2

[0016] Example 2 Catalytic reaction: put 90% pure maltose into maltose syrup with a concentration of 20% and put it into the reaction tank (1), set the temperature of the reaction solution at 34-36°C, and add the The trehalose synthase of Streptomyces griseus CCTCC AA92094 catalyzes the reaction, and the optimum pH range of the trehalose synthase is 7.2-7.8.

Embodiment 3

[0017] Example 3 Actual pH measurement: Time-sharing sampling was carried out for the two catalytic reactions without pH adjustment and pH adjustment respectively in the reaction process, and the pH value of each sample was measured in real time with a pH meter. The results are shown in Table 2.

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Abstract

The invention discloses a monitoring method for the production of trehalose by a single enzyme method. First, a near-infrared real-time monitoring and control system is installed, samples are collected, and the pH values ​​measured in real time by pH meters of each sample correspond to the pH values ​​measured by high-performance liquid chromatography of each sample. Data such as trehalose content are correlated with the near-infrared spectrum information of each sample collected correspondingly, and a quantitative model of the change of trehalose content and pH value per unit time in the single-enzyme production process is established and corrected. The established quantitative calibration model is used to quickly detect the change of trehalose content and pH value per unit time in the single-enzyme production process, and the control system is used to stabilize the pH value within the optimal range of trehalose synthase activity. The method of the invention can not only monitor the reaction liquid in the single-enzyme production process in real time, but also automatically control the pH value and the change of the trehalose content, and the result is accurate and reliable.

Description

technical field [0001] The present invention belongs to an invention about trehalose, more specifically, the present invention relates to a method for monitoring and controlling the production of trehalose by a single enzyme method. Background technique [0002] Trehalose is a non-reducing disaccharide composed of two molecules of glucose connected by α, α-1, 1-glycosidic bonds. The large-scale production methods of trehalose” and “new sugars in the 21st century” have received widespread attention; the production methods of trehalose include natural biological extraction, microbial fermentation, chemical synthesis, genetic engineering and enzymatic conversion; At present, industrial production basically adopts enzymatic conversion method, which is mainly divided into single-enzyme method and double-enzyme method; in the production process of trehalose by single-enzyme method, trehalose synthase converts maltose into trehalose, and the nature of trehalose synthase determines ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G05D21/02G01N21/359G01N21/3577
CPCG01N21/3577G01N21/359G05D21/02
Inventor 谢定郑瑞娜杨倩圆易翠平
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY