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A method for preparing helical dextrin inclusion complex carrier by alcohol precipitation and classification

A technology of inclusion complexation and carrier, which is applied in the preparation of helical dextrin inclusion complexation carrier by alcohol precipitation and classification, and in the field of preparation of helical dextrin by combining biotechnology and chemical methods, which can solve the problem of large molecular weight of amylose and the reduction of inclusion complexation. Embedding efficiency, reducing the content of V-type crystal complexes, etc., to achieve the effect of simple preparation process, improved embedding rate, and less by-products

Active Publication Date: 2021-06-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The molecular weight of amylose is large, the chain length is too long, and more chains do not interact with guest molecules to form embeddings, but the amylose chains are easy to interact with each other to form aged starch, which reduces the V-type crystal complex. content, which reduces the efficiency of forming inclusions with guest molecules

Method used

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  • A method for preparing helical dextrin inclusion complex carrier by alcohol precipitation and classification
  • A method for preparing helical dextrin inclusion complex carrier by alcohol precipitation and classification
  • A method for preparing helical dextrin inclusion complex carrier by alcohol precipitation and classification

Examples

Experimental program
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Embodiment 1

[0025] The preparation of embodiment 1 helical dextrin

[0026] In the first step, raw corn starch is formulated with acetic acid-sodium acetate buffer solution to form starch milk with a mass percentage concentration of 5%, and the pH is adjusted to 6.5, and placed in a closed container at 99°C for 60 minutes to gelatinize; after gelatinization is completed, Cool the gelatinized liquid to 45°C, add isoamylase, the dosage is 7.5U of isoamylase per gram of dry starch (200000U / g, Hubei Hongyunlong Biotechnology Co., Ltd.), stir and react at 55°C for 12h, then heat Inactivate the enzyme at 100°C for 10 minutes, and cool rapidly to obtain the debranched starch solution. The above-mentioned method for determining the debranching rate of debranched starch is used to determine that the starch has been completely debranched, and the obtained debranched starch is all connected by α-1,4 glycosidic bonds without branching parts.

[0027] The second step is to cool the debranched starch ...

Embodiment 2

[0029] The preparation of embodiment 2 helical dextrin

[0030] The first step is to prepare waxy corn raw starch with acetic acid-sodium acetate buffer solution to form starch milk with a mass percentage concentration of 10%, and adjust the pH to 5.5, and place it in a closed container at 95°C for 80 minutes to gelatinize; gelatinization is complete Finally, the gelatinized liquid was cooled to 55°C, and pullulanase was added in an amount of 25 U of pullulanase (OPTIMAX L-1000, Genencor Bioengineering Co., Ltd.) per gram of dry starch, and the reaction was stirred at 45°C After 10 hours, heat to 100°C to inactivate the enzyme for 10 minutes, and cool down rapidly to obtain the helical dextrin solution. The above-mentioned method for measuring the debranching rate of the helical dextrins is used to determine that the starch has been completely debranched, and the obtained helical dextrins are all connected by α-1,4 glycosidic bonds without branching parts.

[0031]In the seco...

Embodiment 3

[0032] The preparation of embodiment 3 helical dextrin

[0033] In the first step, the raw potato starch is formulated with acetic acid-sodium acetate buffer solution to form starch milk with a mass percentage concentration of 10%, and the pH is adjusted to 6.0, and it is placed in a closed container at 98°C for 70 minutes to gelatinize; after gelatinization is completed, Cool the gelatinized liquid to 45°C, add isoamylase, the dosage is 10U of pullulanase (200 000U / g, Hubei Hongyunlong Biotechnology Co., Ltd.) per gram of dry starch, stir and react at 50°C for 11h, then Heat to 100°C to inactivate the enzyme for 10 minutes, then cool rapidly to obtain the helical dextrin solution. The above-mentioned method for measuring the debranching rate of the helical dextrins is used to determine that the starch has been completely debranched, and the obtained helical dextrins are all connected by α-1,4 glycosidic bonds without branching parts.

[0034] In the second step, the spiral d...

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Abstract

The invention discloses a method for preparing a helical dextrin inclusion and complexation carrier by alcohol precipitation and classification, and belongs to the field of chemical, food and pharmaceutical compound carrier materials. In this method, the proamylase is completely debranched to obtain a debranched starch solution; then the debranched starch solution is concentrated by rotary evaporation, and absolute ethanol is slowly added dropwise to the concentrated solution of helical dextrin until the ethanol concentration is 40- 60% (v / v), put it in a refrigerator at 4°C for 12-24 hours, centrifuge, take the precipitate, dry it, and grind it to get the helicoid dextrin product. The operation method of the invention is simple, the condition is mild, and the distribution of product components is narrow. By adopting the method of the invention, the embedding rate of some guest molecules can be effectively improved, and the application range of the starch in the transport carrier can be expanded.

Description

technical field [0001] The invention relates to a helical dextrin carrier, in particular to a method for preparing a helical dextrin inclusion complex carrier by alcohol precipitation and classification, specifically referring to a method for preparing a helical dextrin by combining biotechnology and chemical methods; it belongs to chemical industry, food , The field of production methods of drug embedding materials. Background technique [0002] Helical dextrin is composed of glucose units connected by α-1,4 glycosidic bonds, has a flexible conformation, and its spatial structure has a flexible helical structure. Helical dextrins have a cavity structure similar to amylose and cyclodextrins. Spiral dextrin can be used as the host molecule, and the hydrophobic cavity can form inclusion complexes by inclusion of various inorganic or organic small molecular substances. After the guest molecule is included and complexed, its microenvironment, including physical, chemical, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12P19/16C08B30/18C08B30/20
Inventor 罗志刚王萍萍
Owner SOUTH CHINA UNIV OF TECH
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