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A kind of biocontrol strain ch01 for effectively preventing and treating citrus canker and its application

A technology for citrus canker and biocontrol strains, applied in application, bacteria, biocide and other directions to increase income and reduce burden

Active Publication Date: 2020-04-28
NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main control measures against canker in the citrus production process are still spraying chemical agents, but the long-term use of chemical agents has made the canker bacteria resistant to various fungicides

Method used

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  • A kind of biocontrol strain ch01 for effectively preventing and treating citrus canker and its application
  • A kind of biocontrol strain ch01 for effectively preventing and treating citrus canker and its application
  • A kind of biocontrol strain ch01 for effectively preventing and treating citrus canker and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Sample collection

[0024] CH01 was isolated from the rhizosphere soil of navel orange plants in navel orange orchard in Dingnan County, Jiangxi Province.

[0025] 2) Strain isolation method

[0026] The plate dilution method was used (refer to Microbiology Room of Nanjing Institute of Soil Science, Chinese Academy of Sciences. Soil Microbial Research Method [M]. Beijing: Science Press, 1985).

[0027] 3) Plate antagonism assay

[0028] Pathogenic fungi: using the plate confrontation method: Gibberella, Botrytis cinerea, Fusarium oxysporum specialization type Cuban, Fusarium oxysporum specialization watermelon type, Globula meloni, cultured in an incubator at 25°C for 5 days, marginal Take a 0.6cm-diameter fungus dish, and inoculate Gibberella, Botrytis cinerea, Fusarium oxysporum Cuban specialization, Fusarium oxysporum watermelon specialization, and melon globules in the center of the PDA plate, and then inoculate them in the center of the PDA plate. The four co...

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Abstract

The invention discloses a biocontrol bacterial strain CH01 for efficiently preventing and treating citrus canker and an application of the biocontrol bacterial strain CH01. The biocontrol bacterial strain CH01 is bacillus cereus, and is assigned with the preservation number CGMCC NO.11677. It is shown from a flat inhibition test result the bacillus cereus CH01 can obviously inhibit growth of xanthomonas axonopodis pv. citri, and has an obvious inhibition effect on multiple pathogenic fungi. Further, the bacillus cereus CH01 shows great hydrolase activity in vitro. It is shown from a greenhousetest result that the bacillus cereus CH01 can effectively prevent development of citrus canker, and it is shown from a field trial result that the bacillus cereus CH01 has 65.46% of prevention effecton citrus canker. Therefore, the biocontrol bacterial strain CH01 can be used as a biocontrol preparation and efficiently prevent citrus canker.

Description

technical field [0001] The invention belongs to the technical field of biological control of crop diseases, and relates to a biocontrol strain CH01 for efficiently preventing and treating citrus canker and its application. Background technique [0002] Rutaceae citrus fruits, such as oranges, oranges and lemons, are popular fruits. With the advancement of high-efficiency agriculture, the production of citrus industry in my country has developed rapidly, and the planting area has expanded rapidly. With the specialization of citrus planting, many years of cultivation have increased the base of citrus canker pathogens in the orchard year by year, which has led to the occurrence of citrus canker becoming more and more serious. The general loss rate is 15% to 20%; in severe cases, it reaches 30% to 50%. Up to 100%, causing treetop canker lesions, a large number of leaf disease, fruit peel deformity, ulcers, seriously affecting the appearance and taste of the fruit, and losing its...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00C12R1/085
Inventor 王超席运官田伟黄思杰李妍肖兴基
Owner NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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