Nano chitosan capable of quickly adjusting drug release rate
A nano-chitosan, rapid adjustment technology, applied in the direction of capsule delivery, microcapsules, nanocapsules, etc., can solve the problem of not really displaying the biological activity of chitosan
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[0015] 1.1 Materials
[0016] Chitosan (CS, molecular weight 5000Da, deacetylation degree 85%), Zhejiang Aoxing. DL-Lipoic Acid 99% (LA), Stearic Acid 98% (SA), 1-(3-Dimethylaminopropyl)-3-Ethylcarbodiimide Hydrochloride (EDC), N-Hydroxysuccinate Imide (NHS) and dithiothreitol (DTT) were purchased from Aladdin Reagents (China) Co., Ltd. Formic acid solution (concentration: 88%), formaldehyde solution (concentration: 40%), and absolute ethanol were all of analytical grade and purchased from Tianjin Reagent Wholesale Company.
[0017] 1.2 Preparation of grafted chitosan
[0018] Get 1g chitosan and be dissolved in 70mL deionized water, stearic acid (the mol ratio of the amino group of stearic acid and chitosan is 0.2:1) and 1.6g EDC and 0.95g NHS are dissolved in 35mL ethanol (EDC and The molar ratio of NHS to carboxyl group is 10:1), pre-reacted at room temperature for 0.5h, then added dropwise into chitosan aqueous solution, and reacted at 60°C for 2d. After the reaction, ...
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