A method for measuring β-lactamase in samples

Abstract

The invention provides a method for testing beta-lactamase in a sample. The sample is detected by using a kit for testing beta-lactamase in samples; the kit contains a reaction substrate and a samplediluent, wherein beta-lactamase detection holes are formed in the reaction substrate; a beta-lactamase substrate (such as cephalosporin), a stabilizer, an oxidant and the like are wrapped by the beta-lactamase detection holes. The kit provided by the invention is simple in preparation method, stable in property, easy to preserve, convenient in judging whether a clinical sample has beta-lactamase drug resistance strains or not, is an auxiliary index for diagnosing and identifying diagnosis and treatment effects, is capable of instructing clinical medicine application, is applicable to operatorsof hospital clinical labs and nonprofessional inspectors, and can be also expanded to detection on food environment samples.

Description

technical field [0001] The invention belongs to the technical field of medical examination and determination, and in particular relates to a method for determining β-lactamase in a sample. Background technique [0002] Since the advent of penicillin, the first β-lactam antibiotic in the 1940s, antibiotics have played an important role in the treatment of bacterial infectious diseases, and more than 95% of the diseases caused by bacterial infections have been controlled. However, with the continuous development of β-lactam antibiotics and their wide clinical application, more and more drug-resistant strains, the main resistance mechanism is that these strains can produce β-lactamase. [0003] Studies have shown that β-lactamase is a class of enzymes that hydrolyze the β-lactam ring structure in β-lactam antibiotic molecules. According to the homology of the amino acid sequence of the enzyme protein, it is divided into class A, class B, class C and class D Among them, extende...

AI Technical Summary

Problems solved by technology

[0009] However, the safety of β-lactamase has not been confirmed at present, and the relationship between the products of β-lactam antibiotics decomposed by enzymes and the toxic and side effects of antibiotics has not been studied in depth. Although the addition of β-lactamase in food Can decompose antibiotics, but long-term exposure to antibiotic enzyme decomposition products also has a great risk

[0010] The UscnlifeTM human and chicken β-lactamase enzyme-linked immunoassay kit developed by PANPACIFIC TECH COMPANY of the United States uses an antibody against a β-lactamase, and there are many types of β-lactamase , the structures are different from each other, and the detection reagent prepared after preparing an antibody with a β-lactamase as an antigen cannot detect other types of β-lactamase, which limits the application of detection kits based on the principle of ELISA