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Efficient culture method for photosynthetic bacterial inoculant

A cultivation method and technology for photosynthetic bacteria, applied in the field of valve manufacturing, can solve the problems of low efficiency of natural cultivation of photosynthetic bacteria, and achieve the effect of good quality and high efficiency

Inactive Publication Date: 2018-02-02
成都市新津胤春生物科技有限责任公司
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Problems solved by technology

[0003] However, the efficiency of naturally growing photosynthetic bacteria inoculum is extremely low, and it is necessary to improve the cultivation efficiency of inoculum

Method used

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Embodiment Construction

[0012] The present invention will be further described below in conjunction with specific embodiments.

[0013] A high-efficiency culture method for photosynthetic bacteria inoculum, is characterized in that, comprises the following steps:

[0014] A. Culture medium preparation: prepare pure water to be weakly alkaline, with a pH value of 7.5-8.5, and add 0.2 g of potassium propionate, 0.1 g of sodium chloride, 0.1 g of ammonium chloride, and dihydrogen phosphate into every 100 ml of purified water Potassium 0.2g, yeast extract 0.2g, vitamin 0.1g;

[0015] B. Selection of strains: select effective live strains, and the strains include Rhodopseudomonas sphaericus and Rhodopseudomonas paluscens, mixed according to the mass percentage of 1:1.5; wherein Rhodopseudomonas palustris is divided into double-layer plates Cultivate until a red single colony appears;

[0016] C. Seed liquid culture: Ferment and cultivate the mixed live strains obtained in the above step B, the temperatu...

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Abstract

The invention discloses anefficient culture method for a photosynthetic bacterial inoculant. The method includes the steps of 1, culture medium preparation, wherein purified water is adjusted to be alkalescent, the pH value is 7.5-8.5, and the purified water per 100ml is added with 0.2g of potassium propionate, 0.1g of sodium chloride, 0.1g of ammonium chloride, 0.2g of monopotassium phosphate, 0.2 g of yeast extract and 0.1g of vitamin; 2, strain selection, wherein an effective active strain is selected, and the strain includes rhodopseudomonas spheroides and rhodopseudomonas palustris whichare mixed by mass of 1:1.5, wherein the rhodopseudomonas palustris is cultured by a double-layer plate culture method until red single colonies appear; 3, seed solution culture, wherein the mixed active strain obtained in step 2 is fermented and cultured, the culture temperature is 27-32 DEG C, and the light condition is 2500-3000 Lx. By using the method, the culture efficiency of the photosynthetic bacterial inoculant can be effectively improved.

Description

technical field [0001] The invention relates to the technical field of valve manufacturing, in particular to a method for efficiently cultivating photosynthetic bacteria bacterial agents. Background technique [0002] Photosynthetic bacteria (PSB for short) are prokaryotic organisms that first appeared on the earth, are ubiquitous in nature, and have a primitive light energy synthesis system. Gram-negative bacteria with the ability to use light as energy, can use organic matter, sulfide, ammonia, etc. in nature as hydrogen donors and carbon sources for photosynthesis under anaerobic light or aerobic dark conditions . Photosynthetic bacteria are widely distributed in soil, paddy fields, swamps, lakes, rivers and seas in nature, and are mainly distributed in anoxic areas where light can penetrate in aquatic environments. [0003] However, the efficiency of naturally growing photosynthetic bacteria inoculum is extremely low, and it is necessary to improve the cultivation effi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/38
CPCC12N1/20
Inventor 王品孟黄丽
Owner 成都市新津胤春生物科技有限责任公司
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