Cell retention device
A technology for trapping devices and cells, applied in biochemical instruments, biochemical equipment and methods, and methods for supporting/immobilizing microorganisms, etc., can solve problems such as reducing the service life of diaphragm sheets, replacing hollow fiber columns, and large vibration of diaphragm pumps. , to achieve the effect of prolonging life, low shear force output and improving production efficiency
Pending Publication Date: 2018-02-06
成都赛安普飞科技有限公司
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Problems solved by technology
The existing ATF equipment and TFF equipment cannot realize the replacement of the hollow fiber column without interruption, and the existing ATF equipment relies on compressed air and vacuum air source as the power to drive the diaphragm pump to run. The power is switched from positive pressure compressed air to negative pressure vacuum; when the diaphragm pump runs in reverse, it generates a large vibration, and produces a large instantaneous shear force on the diaphragm and cultured cells, which reduces the use of the diaphragm Lifespan, also increases the possibility of cell damage due to excessive shear stress
Method used
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[0014] The specific implementation manner of the present invention will be illustrated below with reference to the accompanying drawings, and the protection scope of the present invention is not limited to the following description.
[0015] figure 1 It is an example of a filter system with a liquid storage container (bioreactor) represented according to the present invention, with two filters, the two filters operate simultaneously, and the fluid outlet is on the upper part of the hollow fiber filter column.
[0016] The filtration system includes two filters A and B, filter A and filter B have the same effect on fluid filtration; in order to achieve this effect, the two sets of filters A and B can have the same or different structures and components , does not rule out that its components are different in size and specification. For ease of description, figure 1 Indicates one of the cases where filters A and B have the same structure and components.
[0017] The pipeline ...
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The invention discloses a cell retention device. The device comprises a driving device adopting a linear motor to supply power, a switching device and two filters, wherein each filter comprises a hollow fiber filter column and a diaphragm pump; the driving device drives the diaphragm pumps so as to enable fluid containing cells to enter the diaphragm pumps through the hollow fiber filter columns,and the process can be reversed; by means of one switching device, the driving device can only drive one diaphragm pump to operate or drive the two diaphragm pumps to operate simultaneously, and the switching can be manual or automatic; when one diaphragm pump operates, the other filter can perform operation such as consumable replacement, trouble clearing, sterilization and the like, and interruption of production is avoided; besides, diaphragm displacement, diaphragm pump operating speed, accelerated speed and driving moment can be adjusted. The device is simple to operate, operates stably,does not produce instantaneous shear force on cells, can realize uninterrupted production and is high in automation degree and production efficiency.
Description
technical field [0001] The invention relates to a cell retaining device, which belongs to the field of pharmaceutical equipment. Background technique [0002] Interest in continuous processes has been increasing in recent years. The main differentiating advantages of perfusion compared to batch or fed-batch are the shorter residence time of the target product in the bioreactor, higher cell density and corresponding higher daily unit volume yield, longer Culture time, healthier cell status, and better control over the cell growth environment. [0003] In mammalian cell perfusion, cell separation devices can be classified according to different physical principles: filtration, acceleration / gravity, and electric fields. In large-scale cultivation, the main advantage of having separation equipment outside the reactor is that it is easier to operate, especially if the equipment starts to degrade, and can be easily replaced or re-sterilized. [0004] In large-scale suspension c...
Claims
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IPC IPC(8): C12M1/12
CPCC12M23/52C12M23/58C12M29/00C12M33/14
Inventor 黄文卉
Owner 成都赛安普飞科技有限公司