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Use of pentapeptide kttks in the treatment of degenerative arthritis

A technology of degenerative arthritis and five peptides, which is applied in the field of five/hexapeptide KTTKS in the treatment of degenerative arthritis, and can solve the problems that the pathogenic mechanism of degenerative arthritis is not yet clear.

Active Publication Date: 2020-09-18
ADVANCED HEALTHTECH BIOPETIDE LAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although aging is known to affect cartilage lesions, the true pathogenic mechanism of osteoarthritis has not yet been understood

Method used

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  • Use of pentapeptide kttks in the treatment of degenerative arthritis
  • Use of pentapeptide kttks in the treatment of degenerative arthritis
  • Use of pentapeptide kttks in the treatment of degenerative arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Animal model

[0024] Fifteen healthy adult New Zealand white rabbits were randomly divided into 3 groups: normal control group (group A), model control group (group B) and peptide intra-articular injection group (group C), with 5 rabbits in each group. Fasting for 4 hours before the operation, intramuscular injection of Ketamine (10mg / kg) and Atropine (0.2mg / kg). After the anesthesia took effect, the animal was fixed on the operating table, an 8 cm longitudinal incision was made at the posterior edge of the patella ligament and the skin was raised, the joint capsule and synovium were opened along the posterior edge of the patella, and the knee joint was bent 90° to expose the cartilage at the lower end of the femur. Two cylindrical defects with a diameter of 3 mm and a depth of 3 mm were made to remove the coagulation in the defects, and after cleaning the joint cavity with sterile saline, they were carefully sutured in layers. Penicillin was injected for anti-infe...

Embodiment 2

[0042] 1. Experimental method

[0043] Firstly, take rabbit growth plate chondrocytes and carry out continuous culture. Set up pentapeptide KTTKS alone action group, collagen alone action group, pentapeptide KTTKS+collagen joint action group, respectively add pentapeptide KTTKS 250ppm, collagen (100ng / ml) and pentapeptide KTTKS (250ppm) + collagen (100ng / ml), and a negative control group was set up. After the new cartilage tissue covered the bottom of the centrifuge tube, it was transferred to a 24-well culture plate to continue culturing, and the medium was replaced every other day. After the predetermined culture time (3, 7 and 14 days), samples were collected for various tests.

[0044] Next, histological and histochemical tests were performed: specimens were collected for HE staining and immunohistochemical (S-P method) testing.

[0045] Afterwards, the Hoechst 33258 method was used to measure the DNA content of chondrocytes.

[0046] Next, the synthesis of chondrocyte m...

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Abstract

The invention discloses an application of palmitoyl / argireline KTTKS (F) in treating degenerative joint disease.

Description

technical field [0001] The present invention relates to the new use of five / hexapeptide KTTKS (F) in treating degenerative arthritis. Background technique [0002] Degenerative joint disease (DJD) refers to the persistent lesion of articular cartilage accompanied by the hyperplasia of hyperplastic bone, in which the hyperplastic bone mostly occurs at the edge of the joint or under the cartilage. The causes of osteoarthritis are quite complicated, but mainly related to the age of the patient at the time of onset. Osteoarthritis often occurs in older adults. Confirmation of the development of osteoarthritis is based on detection of wear and fibrosis of articular cartilage, development of new bone (bone spurs), and / or soft tissue inflammation. Although it is known that age changes do affect cartilage lesions, the true pathogenesis of osteoarthritis has not yet been understood. [0003] Osteoarthritis mostly occurs in the joints that bear body weight or the interphalangeal jo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/08A61P19/02
CPCA61K38/08
Inventor 庄国升
Owner ADVANCED HEALTHTECH BIOPETIDE LAB CO LTD