White light-emitting up-conversion nanoparticle and test paper strip based on white light-emitting up-converting nanoparticle and capable of simultaneously detecting multi-component tumor markers

A technology of tumor markers and nanoparticles, which is applied in the field of up-conversion test paper detection of multi-component tumor markers, can solve the problems of affecting detection efficiency and complicated manufacturing process, and achieve good safety, high accuracy and specificity, The effect of removing background distractions

Active Publication Date: 2018-03-02
HEFEI UNIV OF TECH
View PDF9 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, when the existing up-conversion test strips detect multiple tumor markers at the same time, different T-lines need to

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • White light-emitting up-conversion nanoparticle and test paper strip based on white light-emitting up-converting nanoparticle and capable of simultaneously detecting multi-component tumor markers
  • White light-emitting up-conversion nanoparticle and test paper strip based on white light-emitting up-converting nanoparticle and capable of simultaneously detecting multi-component tumor markers
  • White light-emitting up-conversion nanoparticle and test paper strip based on white light-emitting up-converting nanoparticle and capable of simultaneously detecting multi-component tumor markers

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0050] Example 1. Detection of CEA and AFP tumor markers

[0051] This embodiment takes CEA and AFP as the tumor markers to be detected as an example, and the details are as follows.

[0052] 1. Preparation of UCNPs-antibody

[0053] 1.1. Prepare white luminescent upconversion nanoparticles with a three-layer core-shell structure by seeding method, which is based on NaGdF doped with Yb, Tm and Er 4 Nanoparticles are the inner core, and Eu doped NaGdF is wrapped around the inner core 4 The first shell, and NaYF is wrapped around the first shell 4 The second shell layer constitutes NaGdF co-doped with Yb, Tm, Er and Eu 4 :Yb / Tm / Er@NaGdF 4 :Eu@NaYF 4 Core-shell structure; the specific preparation steps are as follows:

[0054] a. Synthesis of NaGdF doped with Yb, Tm and Er 4 Nanoparticles as the core

[0055] Weigh 10 mL of oleic acid (OA), 10 mL of octadecene (ODE), and Gd (OAc) 3 0.0841g, Yb(OAc) 3 0.0858g, 25μL of 0.1mol / L Tm aqueous solution, 2μL of 0.2mol / L Er aqueous solution, and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Lengthaaaaaaaaaa
Lengthaaaaaaaaaa
Login to view more

Abstract

The invention discloses a white light-emitting up-conversion nanoparticle and a test paper strip based on the white light-emitting up-converting nanoparticle and capable of simultaneously detecting multi-component tumor markers. According to the present invention, the nanoparticle has a three-layer core-shell structure, and emits white light under the excitation of near infrared light; and after the antibodies of multi-component tumor markers to be detected are labeled with the nanoparticle, the obtained conjugates are immobilized on the labeling complex binding zone of the test paper strip, such that the multiple tumor markers can be simultaneously detected; and with the nanoparticle and the test paper strip, the sensitive detection on the multiple tumor markers by the single up-conversion nanoparticle is achieved, and the advantages of simple operation, good specificity, high qualitative and quantitative real-time detection and high sensitivity are provided.

Description

technical field [0001] The invention belongs to the technical field of biomedical diagnosis, and in particular relates to an up-conversion test paper detection method for multi-component tumor markers. Background technique [0002] Malignant tumors are often referred to as cancer. The expression of some genes in the body is out of control. The malignant proliferation of cells destroys the normal life activities of the body. Its growth speed is fast, which will cause weight loss, fever, and serious organ damage. The final crisis life. At present, cancer has become the number one killer threatening people's lives. Therefore, early detection of cancer and timely clinical monitoring have become a major issue in modern biomedicine. With the maturity of nanotechnology, the combination platform of nanotechnology and cancer markers for the detection of tumor markers has become a hot spot. Colloidal gold test strips are the earliest widely used immunochromatographic test strips. I...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/63
CPCG01N21/63
Inventor 邓胜松高梦萍梅青松
Owner HEFEI UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap