Saccharifying technique for ungerminated barley
A barley and technology technology, applied in the field of beer brewing, can solve the problems of high raw material price, large energy consumption, cumbersome malting process, etc.
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Embodiment 1
[0018] 1. Through the staining of barley endosperm starch with iodine-potassium iodide reagent and the determination of amylose content, select waxy barley with 3.5% amylose content.
[0019] 2. The protein content and β-glucan content of the waxy barley were determined to be 12% and 6.6% respectively.
[0020] 3. Cleaning, removing impurities, drying, and pulverizing to obtain coarse powder passing through a 50-mesh sieve;
[0021] 4. The water ratio of the total saccharification material is 1:4.5. Add water to the saccharification pot, preheat it to 45°C, add neutral protease, β-glucanase, xylanase, and α-amylase respectively. The mass ratios of ungerminated waxy barley flour were 250:1, 125:1, 1000:1, 250:1, respectively, and the pH was adjusted to 5.4.
[0022] 5. Keep the mash tun at 45°C and stir for 30 minutes, raise the temperature to 70°C (1°C / min), keep warm and stir for 60 minutes, cool to 20°C, and filter to obtain easy-to-filter wort.
[0023] 6. Evaluate the qu...
Embodiment 2
[0025] 1. Through the dyeing of barley endosperm starch with iodine-potassium iodide reagent and the determination of amylose content, select waxy barley without amylose.
[0026] 2. The protein content and β-glucan content of the waxy barley were determined to be 12% and 6.6% respectively.
[0027] 3. Cleaning, removing impurities, drying, and pulverizing to obtain coarse powder passing through a 50-mesh sieve;
[0028] 4. The water ratio of the total saccharification material is 1:4.5. Add water to the saccharification pot, preheat it to 45°C, add neutral protease, β-glucanase, xylanase, and α-amylase respectively. The mass ratios of ungerminated waxy barley flour were 250:0.5, 125:0.5, 1000:0.5, 250:0.5, and the pH was adjusted to 5.3.
[0029] 5. Keep the mash tun at 45°C and stir for 30 minutes, raise the temperature to 70°C (1°C / min), keep warm and stir for 60 minutes, cool to 20°C, and filter to obtain easy-to-filter wort.
[0030] 6. Evaluate the quality of the obtai...
Embodiment 3
[0032] 1. Through the dyeing of barley endosperm starch iodine-potassium iodide reagent and the determination of amylose content, select high-quality waxy barley with 14% amylose content.
[0033] 2. The protein content and β-glucan content of the waxy barley were determined to be 15% and 9.2%, respectively.
[0034] 3. Cleaning, removing impurities, drying, and pulverizing to obtain coarse powder passing through a 50-mesh sieve.
[0035] 4. The water ratio of the total saccharification material is 1:4.5. Add water to the saccharification pot, preheat it to 45°C, add neutral protease, β-glucanase, xylanase, and α-amylase respectively. The mass ratios of ungerminated waxy barley flour were 250:1.5, 125:1.5, 1000:1.5, 250:1.5, and the pH was adjusted to 5.5.
[0036] 5. Keep the mash tun at 45°C and stir for 30 minutes, raise the temperature to 70°C (1°C / min), keep warm and stir for 60 minutes, cool to 20°C, and filter to obtain easy-to-filter wort.
[0037] 6. Evaluate the qu...
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