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A kind of nickel cholinesterase electrode embedded in infiltrating foam and its application

A technology of cholinesterase and acetylcholinesterase, which is applied in the direction of material electrochemical variables, material analysis by electromagnetic means, instruments, etc. Resistance and other problems, to achieve the effect of good stability, fast response, high sensitivity detection

Active Publication Date: 2020-10-02
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent ZL 201310042627.9 (Xie Deming, Liu Yong, Xue Yongliang, Yang Yanping. Cholinesterase electrode with nickel foam as carrier and its application) discloses a cholinesterase electrode with nickel foam / graphite as carrier, but it is in What used in the preparation of foamed nickel / graphite carrier is oily binder, thus there are following problems: (1) use organic solvent, not environmental protection; Or the active material is mixed with a binder to make a slurry, filled into the skeleton, and then dried and pressed to form an electrode
The problem with this technology is that the slurry has to contain a binder to reduce the occupancy of the conductive material or active material, which directly reduces the increase in internal resistance, slows down the reaction speed, and absorbs the electrolyte.
The dry powder filling technology has not been applied to the cholinesterase electrode for the detection of pesticides so far

Method used

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  • A kind of nickel cholinesterase electrode embedded in infiltrating foam and its application
  • A kind of nickel cholinesterase electrode embedded in infiltrating foam and its application
  • A kind of nickel cholinesterase electrode embedded in infiltrating foam and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the preparation of the embedded impregnation (chitosan) acetylcholinesterase electrode of carrier with nickel foam / graphite

[0043] 1) Graphite-iron phosphide mixed powder filling: After mixing 500 mesh graphite and 600 mesh iron phosphide in a mass ratio of 80:20, add 2% chitosan solution dropwise (addition amount is graphite-iron phosphide mixed powder 4% of the quality) is filled in the foamed nickel plate with embedding mode after stirring, and the floating powder is scraped off with a scraper to obtain an unformed foamed nickel / graphite carrier layer; wherein

[0044] Configuration of 2% chitosan solution: add chitosan into 0.5% dilute acetic acid aqueous solution according to the metering ratio to fully dissolve, and place it in a refrigerator at 4°C for later use.

[0045] 2) Preparation of foamed nickel / graphite carrier layer: Press the unformed foamed nickel / graphite carrier layer obtained in 1) into a sheet with a thickness of 0.5mm under a roll...

Embodiment 2

[0054] Embodiment 2: Embedded (chitosan) acetylcholinesterase electrode is used for the electrochemical detection of organophosphorus and carbamate pesticides

[0055] Step 1) to step 6) are the same as in Example 1.

[0056] 7) Preparation of chlorpyrifos standard sample: take 0.731g chlorpyrifos pesticide stock solution, dissolve it in 100ml of 1.2mmol / L acetylcholine chloride phosphate buffer solution (pH7, containing 0.10mol L -1 In KCl), configure the pesticide concentration to be 1.0×10 -2 mo1 / L detection solution, and then gradually diluted to a pesticide concentration of 1.0×10 -4 mo1 / L, 1.0×10 -5 mo1 / L, 1.0×10 -6 mo1 / L, 1.0×10 -7 mo1 / L, 1.0×10 -8 mo1 / L, 1.0×10 -9 mo1 / L detection solution.

[0057] 8) Take the prepared acetylcholinesterase electrode as a working electrode, use a platinum electrode as a counter electrode, and Ag / AgCl as a reference electrode, add 1mmoL / L of thioacetylcholine chloride in a phosphate buffered saline buffer solution (PBS, pH value ...

Embodiment 3

[0061] The stability of embodiment 3 embedding (chitosan) acetylcholinesterase electrode

[0062] After the acetylcholinesterase electrode prepared according to the method of Example 1 was stored at 4°C for 30 days, the oxidation current of acetylcholine chloride was measured according to the method of Example 2, and the response signal was 88.2% of the original (attached image 3 ). This shows that the acetylcholinesterase electrode has good stability.

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Abstract

The invention discloses a cholinesterase electrode using foamed nickel filled with graphite as a carrier in an embedded and seeped manner. The electrode comprises a foamed nickel / graphite carrier layer and an acetylcholin esterase working layer. The preparation method of an acetylcholin esterase electrode comprises the following steps: (1) after mixing graphite with a trace binder water solution,filling the mixture into a foamed nickel plate in the embedded and seeped manner, scraping floating powder to be crushed into a sheet so as to obtain the foamed nickel / graphite carrier layer; (2) preparing the acetylcholin esterase working layer on the foamed nickel / graphite carrier layer so as to obtain the acetylcholin esterase electrode by using foamed nickel / graphite as the carrier. Compared with the prior art, the cholinesterase electrode has the beneficial effects that firstly, an organic solvent is not used, so that the environment is protected; the foamed nickel plate does not need tobe pretreated, and the foamed nickel / graphite carrier does not need to be dried neither, therefore, the energy is saved; secondly, the cholinesterase electrode has the characteristics of high conductivity, large specific surface area, high response speed, high stability, high detection sensitivity and the like, and can realize high-sensitivity detection for organophosphorus and carbamates pesticide molecules.

Description

technical field [0001] The invention relates to the field of electrochemical biosensors, in particular to an acetylcholinesterase electrode with graphite-filled foam nickel as a carrier and its application in the detection of organic phosphorus and carbamate pesticides. Background technique [0002] Electrochemical biosensors have high selectivity and sensitivity, can respond quickly, and are easy to operate. They are suitable for on-site detection of pesticides, and their research has attracted more and more attention. [0003] Nickel foam is an ideal material for the preparation of electrochemical sensor electrodes. Nickel foam has the characteristics of excellent electrical conductivity, large specific surface area, porous structure, and easy surface modification. It is widely used in battery electrode materials, electrochemical supercapacitors, catalyst carriers, and electrochemical electrode materials. Chinese patent ZL 201310042627.9 (Xie Deming, Liu Yong, Xue Yonglia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/307G01N27/308G01N27/3277
Inventor 谢德明陈晨杨珊珊
Owner ZHEJIANG UNIV OF TECH