Quick high-flux detection method for biological genetic toxicity of combined pollutant in drinking water

Abstract

The invention relates to a quick high-flux detection method for biological genetic toxicity of a combined pollutant in drinking water, belonging to the field of environment detection and evaluation technologies. According to the method, salmonella typhimurium preserved at -20 DEG C is cultivated in a recovery solution for 3 hours, after the absorbance is measured, toxic substances are exposed for3 hours in a micropore board directly; CPRG is selected as an enzymatic substrate, after 30 minutes of reaction, the absorbance is measured. The positive response value of the standard toxic substances is stabilized at 0.015mg.L<-1>, and a standard deviation is 0.00178mg.L<-1>. A bacterial strain is preserved at -20 DEG C, preservation steps of bacteria strains are simplified, the strains are lessdifficult to preserve, and the strain activity is improved. In the experiment, the bacteria strain recovery and pre-cultivation steps are combined and the reagent is optimized, the time is limited within 8 hours, the flexibility and stability are improved. The method is particularly suitable for high-flux detection of biological toxicity of a water sample by non-professional operators, thereby having a bright promotion prospect.

Description

technical field [0001] The invention relates to a rapid and high-throughput detection method for biological genotoxicity of complex pollutants in drinking water, which belongs to the technical category of environmental detection and evaluation. Background technique [0002] Drinking water is a necessity for human beings, and water quality directly affects human health. At present, there are more than 13 million chemical substances that have been synthesized or identified in the world, and as many as 80,000 are common or in use. Among them, carcinogenic substances, such as polycyclic aromatic hydrocarbons (benzopyrene, coal tar, etc.), aromatic amines and other chemical pollutants, and disinfection by-products such as nitrosamines and nitrosamides, etc., are extremely high in drinking water. Low and exists in a mixture state. The detection of a single indicator based on chemical instruments has limitations and cannot directly reflect the genotoxic effect on organisms. Ther...

AI Technical Summary

Problems solved by technology

[0005] But the traditional umu test method still has deficiencies

The traditional method of storing strains at -80°C in ultra-low temperature conditions cannot meet this requirement in many industries, which greatly limits the application and promotion of the method; the traditional method requires recovery and pre-cultivation of the strains, which takes nearly 20 hours, prolonging the Test time; in the international standard ISO13829, the reagent used in the process of breaking the cell wall of the bacterial strain is large in volume and variety, which is likely to cause experimental errors; in the method specified in the international standard, the ONPG solution is used as the substrate for the enzymatic reaction, and the test results show that the cell is The positive response value of toxic substances is low, which reduces the accuracy of experimental data; in addition, in emergency water quality monitoring, traditional methods are time-consuming and labor-intensive, and cannot cope with the high-throughput testing of toxicity of a large number of samples

Images