Determining method of sulfachloropyrazine sodium residue in florfenicol solution
A technology of sulfachloropyrazine sodium and florfenicol is applied in the directions of measuring devices, instruments, scientific instruments and the like to achieve the effects of short detection period, high sensitivity and simple detection method
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[0035] Preparation of Florfenicol Solution:
[0036] Sample 1, the Florfenicol solution prepared by the method of Example 1 disclosed in CN105796488A;
[0037] Sample 2, the Florfenicol solution prepared by the method of Example 2 disclosed in CN105125483A.
[0038] The assay method of residual amount of sulfachlorpyrazine sodium in Florfenicol solution of the present invention comprises the following steps:
[0039] Preparation of standard stock solution: Accurately weigh the sulfachlorpyrazine sodium standard substance, dissolve it in acetonitrile, prepare a standard stock solution with the same concentration as 1 mg / L, and store it at -20°C (the validity period is 1 month); Before use, accurately measure the standard stock solution, dilute it with the mobile phase into a series of standard working solutions of 10ng / ml, 20ng / ml, 50ng / ml, 100ng / ml, 200ng / ml and 500ng / ml, and keep away from it at 4°C. Store in light and use on the same day; preparation of mobile phase: aceto...
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