A method for constructing a liquid-liquid interface electrochemiluminescence system
A construction method, electrochemical technology, applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, analysis by making materials undergo chemical reactions, etc., can solve unfavorable detection applications, poor stability and reproducibility, and low luminous efficiency To avoid mutual interference, improve sensitivity and detection limit
Active Publication Date: 2020-10-16
NORTHWEST NORMAL UNIVERSITY
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Problems solved by technology
Although these luminescent agents have strong and stable electrochemiluminescence signals in organic solutions, the organic phase is not conducive to the actual detection application of electrochemiluminescence systems and pollutes the environment.
If these poorly water-soluble luminescent agents are used in aqueous electrochemiluminescent systems, their luminous efficiency will be particularly low, which is also not conducive to practical detection applications.
Although some chemical modifications and chemical modifications can improve the water solubility and luminous efficiency of the luminescent agent, the process is extremely complicated, and the stability and reproducibility are poor.
Method used
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Embodiment 1
[0029] A method for constructing a liquid-liquid interface electrochemiluminescence system, the specific method is as follows:
[0030] (1) The glassy carbon electrode is sequentially coated with 0.3 μm and 0.05 μm Al 2 o 3 The slurry was ground and then rinsed sequentially with double distilled water and ethanol.
[0031] (2) Dry the surface of the glassy carbon electrode thoroughly with a heat gun (temperature set at 80°C), and then tightly wrap a transparent polytetrafluoroethylene tube with a volume of 200uL and diameters of 5mm and 3mm at both ends on the glassy carbon electrode. the front end of the electrode.
[0032] (3) The luminescent agent tetraphenylporphyrin (TPP) and the co-reactant tetrabutylammonium hexafluorophosphate (TBAPF 6 ) was dissolved in the organic phase 1,2-dichloromethane, the concentration of TPP was 10 -4 mol / L, TBAPF 6 The concentration of the electrode is 0.3mol / L, and the front end of the modified electrode is facing up, and then the lumin...
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The invention discloses a method for constructing a novel liquid-liquid interface electrochemiluminescence system. The method comprises tightly sleeving the front end of a glassy carbon electrode witha Teflon tube, adding an organic solution containing a luminescent agent tetraphenyl porphyrin and a co-reactant tetrabutylammonium hexafluorophosphate into the Teflon tube drop by drop and immersingthe glassy carbon electrode in a water phase containing potassium chloride. The liquid-liquid interface electrochemiluminescence system is easy to construct, utilizes a few of a luminescent agent andproduces an ECL signal with good stability and reproducibility. The ECL signal intensity of the TPP in the system is about twice that in a pure organic phase and luminous efficiency is high. The system can utilize a liquid-liquid interface to divide the luminescent agent and an object to be detected so that mutual interference is avoided. The method realizes the detection of the substance in thefull pH environment. According to the interference of polarity of substances to be detected, the system can extract and enrich the substances to be detected and then carries out quantitative detectionand thus the system can improve the sensitivity and detection limit of material detection.
Description
technical field [0001] The invention belongs to the technical field of electrochemiluminescence system construction, and in particular, relates to a method for constructing a liquid-liquid interface electrochemiluminescence system. The liquid-liquid interface is applied to the electrochemiluminescence system, and a liquid-liquid interface electrochemiluminescence system is constructed. Luminous system. Background technique [0002] In the electrochemiluminescent system, commonly used luminescent agents are all hydrophobic. Although these luminescent agents have strong and stable electrochemiluminescence signals in organic solutions, the organic phase is not conducive to the actual detection application of electrochemiluminescence systems and pollutes the environment. If these luminescent agents with poor water solubility are used in aqueous electrochemiluminescent systems, their luminous efficiency will be particularly low, which is also unfavorable for practical detection ...
Claims
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IPC IPC(8): G01N21/76G01N27/30G01N27/36
CPCG01N21/76G01N27/305G01N27/308G01N27/36
Inventor 卢小泉蒲贵强张东旭王泽汪天胜
Owner NORTHWEST NORMAL UNIVERSITY