Jatrophane type diterpenoid and preparation method and application
A technology of elemane and diterpene, applied in the field of medicine, can solve problems such as increased drug efflux, formation of drug resistance, and inability to speculate on the biological activity of new compounds
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Embodiment 1
[0036] a. Take 10 kg of the fruit of Euphorbia euphorbia, crush it, and use 50 L of 50% ethanol-water solution to extract it by cold soaking at room temperature, and evaporate the solvent to dryness under reduced pressure to obtain the crude extract of Euphorbia ipsifolia;
[0037] b. Disperse the crude extract obtained in step a with ethanol, add petroleum ether for extraction, combine the ethanol layers and evaporate to dryness under reduced pressure to obtain the ethanol extract extract;
[0038] c, the ethanol extract extract obtained in step b is separated with a normal phase silica gel column, and gradient elution is carried out with petroleum ether-ethyl acetate with a volume ratio of 10:1-0:1, and the fraction is subjected to silica gel thin layer chromatography ( TLC) analysis, the same fractions were combined to obtain 6 fractions (F1-F6); fraction F4 was subjected to normal-phase silica gel column separation, and gradient elution was carried out with chloroform-aceto...
Embodiment 2
[0041] a. Take 10 kg of the fruit of Euphorbia euphorbia, crush it, and use 60 L of ethanol-water solution with a concentration of 99% to reflux and extract at a temperature of 80° C., evaporate the solvent under reduced pressure to obtain the crude extract of Euphorbia ipsifolia;
[0042] b. Disperse the crude extract obtained in step a with ethanol, add n-hexane for extraction, combine the ethanol layers and evaporate to dryness under reduced pressure to obtain the ethanol extract extract;
[0043] c, the ethanol extract extract obtained in step b is separated with a normal phase silica gel column, and gradient elution is carried out with n-hexane-ethyl acetate with a volume ratio of 10:1-0:1, and the fraction is subjected to silica gel thin layer chromatography ( TLC) analysis, the same fractions were combined to obtain 6 fractions (F1-F6); fraction F4 was subjected to normal phase silica gel column separation, and gradient elution was carried out with chloroform-methanol wi...
Embodiment 3
[0045]a. Get 10kg of the fruit of Euphorbia euphorbia, pulverize it and extract it by percolation at room temperature with 80L of dehydrated ethanol, and evaporate the solvent to dryness under reduced pressure to obtain the crude extract of Euphorbia ipsifolia;
[0046] b. Disperse the crude extract obtained in step a with ethanol, add cyclohexane for extraction, combine the ethanol layers and evaporate to dryness under reduced pressure to obtain the ethanol extract extract;
[0047] c. Separate the ethanol extract extract obtained in step b with a normal-phase silica gel column, carry out gradient elution with cyclohexane-ethyl acetate with a volume ratio of 10:1-0:1, and fractionate through silica gel thin-layer chromatography (TLC) analysis, the same fractions were combined to obtain 6 components (F1-F6); the component F4 was subjected to normal phase silica gel column separation, and the volume ratio was 100:0-0:100 with dichloromethane-acetone Gradient elution to obtain c...
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