Composite potassium-dissolving bacterial agent for tobacco and its preparation method and application

A technology of compound potassium-dissolving bacteria agent and potassium-dissolving bacteria, which is applied in the field of microorganisms, can solve problems such as poor potassium-dissolving effect, achieve good potassium-dissolving effect, promote root growth, and increase the effect of available potassium content

Active Publication Date: 2019-10-08
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are various types of refractory potassium in soil, and it is difficult for a single strain to have a high diss

Method used

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  • Composite potassium-dissolving bacterial agent for tobacco and its preparation method and application
  • Composite potassium-dissolving bacterial agent for tobacco and its preparation method and application
  • Composite potassium-dissolving bacterial agent for tobacco and its preparation method and application

Examples

Experimental program
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Effect test

Example Embodiment

[0035] Example 1

[0036] Using potassium-dissolving selective medium and tobacco lectin dual screening method, 20 strains of potassium-dissolving strains with specific affinity to tobacco were selected from the tobacco rhizosphere, and 6 strains with better growth-promoting properties were selected to determine their resistance to different difficulties. Solubility of dissolved potassium. The method is: preparing the bacterial suspension of each strain, and inserting it into a potassium dissolving medium containing illite, kaolin, montmorillonite, and potassium feldspar with an inoculum of 2%. The medium formula is Na 2 HPO 4 2.0g, MgSO 4 .H 2 O 0.5g, FeCl 3 0.005g, CaCO 3 0.1g, 1.0g potassium minerals, 5.0g sucrose, 15g agar powder, 1L distilled water, pH7.0-7.5. Cultured on a shaker at 28°C and 160r / min for 5 days. Each Erlenmeyer flask is connected to 4mL H 2 O 2 Digestion at 121℃ for 30min, centrifuge at 6000r / min for 5min, take the supernatant to a constant volume of 100...

Example Embodiment

[0041] Example 2

[0042] S1. Strain activation, connect the potassium-dissolving bacteria TK35 and the potassium-dissolving bacteria TK55 to the LB slant medium, and cultivate for 18h at 25°C to activate the bacteria;

[0043] S2, shake flask culture, wash the slant strains of potassium-dissolving bacteria TK35 and potassium-dissolving bacteria TK55 with physiological saline respectively, and put the washed and activated strains into shake flasks containing LB medium. The volume of each shake flask is 80mL, at a temperature of 25°C and a shaker speed of 120r / min, shake culture for 24 hours to obtain a culture of potassium-dissolving bacteria TK35 and a potassium-dissolving bacteria TK55; in each of the above shake flasks, control the number of viable bacteria at 1 ×10 8 -9×10 8 Within the range of CFU / mL;

[0044] S3. Culture in a fermenter. Connect the potassium-dissolving bacteria TK35 culture obtained in S2 to fermentor A with a 2% inoculum amount, and connect the potassium-diss...

Example Embodiment

[0046] Example 3

[0047] S1. Activation of the strains, insert the potassium-dissolving bacteria TK35 and the potassium-dissolving bacteria TK55 into the LB slant medium, and cultivate at 28°C for 24 hours to activate the strains

[0048] S2, shake flask culture, wash the slant strains of potassium-dissolving bacteria TK35 and potassium-dissolving bacteria TK55 with physiological saline respectively, and put the washed and activated strains into shake flasks containing LB medium. The volume of each shake flask is 100mL, at a temperature of 28°C and a shaker speed of 160r / min, shake culture for 36 hours to obtain a culture of potassium-dissolving bacteria TK35 and a potassium-dissolving bacteria TK55; in each of the above shake flasks, control the number of viable bacteria at 1 ×10 8 -9×10 8 Within the range of CFU / mL;

[0049] S3. Fermenter culture. Connect the potassium-dissolving bacteria TK35 culture obtained in S2 to fermentor A with 5% inoculum, and connect the potassium-disso...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a compound potassium dissolving microbial agent special for tobacco and a preparation method and applicationthereof. The compound potassium dissolving microbial agent is prepared by fermenting and then mixing potassium dissolving bacterium TK35 and potassium dissolving bacterium TK55, the classification name of the potassium dissolving bacterium TK35 is pseudomonas azotoformans which is collected at the China Center for Type Culture Collection on 11th, March 2015 under the collection number of CCTCC M2015098, and the classification name of the potassium dissolving bacterium TK55 is pseudomonas which is collected at the China Center for Type Culture Collection on 30th, Oct. 2017 under the collection number of CCTCC M 2017638. The compound potassium dissolving microbial agent has the advantages that the microbial agent can be in agglutination reaction with tobacco agglutinin, and the tobacco agglutinin mediates planting of TK35 and TK55 strains at roots of tobacco and promotes the strains to stably play a role in promoting tobacco growth.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a composite potassium-dissolving bacterium agent for tobacco and its preparation method and application. Background technique [0002] Tobacco is a potassium-loving economic crop. Potassium can significantly improve the appearance quality of tobacco such as elasticity, softness and color, as well as the combustion performance of tobacco leaves such as burning ability and fire-holding ability, and is an important factor affecting tobacco yield and quality. The potassium content of high-quality tobacco leaves is required to reach more than 2.5%, while the average potassium content of flue-cured tobacco in my country is less than 2%. [0003] In order to improve the quality of tobacco leaves and increase the potassium content of tobacco, potassium fertilizers in the form of potassium sulfate and potassium nitrate are often used in tobacco planting. However, the applic...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/04C05G3/00C05F11/08C12R1/38C05G3/80
CPCC05F11/08C05G3/00C05G3/80C12N1/20
Inventor 曹媛媛郭婷婷何相怡赵为容唐欣昀邓瑞宁
Owner ANHUI AGRICULTURAL UNIVERSITY
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