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Acclimatization method for pleurotus ostreatus bacteria under caustic environment

A strain and technology of Pleurotus ostreatus, applied in botany equipment and methods, mushroom cultivation, application, etc., can solve the problems of edible fungus Pleurotus ostreatus planting yield and quality impact, and achieve the goal of inhibiting the growth of miscellaneous bacteria and improving planting efficiency Effect

Inactive Publication Date: 2018-03-27
翔天科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most edible fungi survive in neutral medium, with a pH of about 6.5, but very miscellaneous fungi are suitable for survival in an acidic environment, which has a great impact on the yield and quality of edible fungi, especially oyster mushrooms.

Method used

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  • Acclimatization method for pleurotus ostreatus bacteria under caustic environment
  • Acclimatization method for pleurotus ostreatus bacteria under caustic environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Include the following steps,

[0018] (1) Adjust the pH value of the medium inoculated with Pleurotus ostreatus to 6.5 with lime water, cultivate for 15 days, and keep the temperature at 28°C to form mycelium;

[0019] (2) Transfer the mycelium formed in step (1) to a medium with a pH value of 8 through a transfer tube, culture for 13 days, and keep the temperature at 26°C to form mycelia; the pH value can be adjusted with lime water;

[0020] (3) Use a transfer tube to transfer the mycelia transfer tube formed in step (2) to a medium with a pH of 9, cultivate for 15 days, and keep the temperature at 25°C to form mycelia; the pH value can be adjusted with lime water;

[0021] (4) Use a transfer tube to transfer the mycelia transfer tube formed in step (3) to a medium with a pH of 10, culture for 11 days, and keep the temperature at 27°C to form mycelia; the pH value can be adjusted with lime water;

[0022] (5) Use a transfer tube to transfer the hyphae formed in step ...

Embodiment 2

[0030] Include the following steps,

[0031] (1) Adjust the pH value of the medium inoculated with Pleurotus ostreatus to 6.3 with lime water, cultivate for 13 days, and keep the temperature at 25°C to form mycelium;

[0032] (2) Transfer the hyphae formed in step (1) to a medium with a pH of 7 through a transfer test tube, culture for 10 days, and keep the temperature at 25°C to form mycelia; the pH value can be adjusted with lime water;

[0033] (3) Use a transfer tube to transfer the mycelia transfer test tube formed in step (2) to a medium with a pH of 8.8, culture for 11 days, and keep the temperature at 26°C to form mycelia; the pH value can be adjusted with lime water;

[0034] (4) Use a transfer tube to transfer the mycelia transfer test tube formed in step (3) to a medium with a pH of 9.5, cultivate for 15 days, and keep the temperature at 28°C to form mycelia; the pH value can be adjusted with lime water;

[0035] (5) Use a transfer tube to transfer the hyphae forme...

Embodiment 3

[0038] Include the following steps,

[0039] (1) Adjust the pH value of the medium inoculated with Pleurotus ostreatus strains to 6 with lime water, cultivate for 10 days, and keep the temperature at 26°C to form mycelium;

[0040] (2) Transfer the hyphae formed in step (1) to a medium with a pH of 7.6 through a transfer test tube, culture for 15 days, and keep the temperature at 28°C to form mycelia; the pH value can be adjusted with lime water;

[0041] (3) Use a transfer tube to transfer the mycelia transfer test tube formed in step (2) to a medium with a pH of 8.5, culture for 14 days, and keep the temperature at 28°C to form mycelia; the pH value can be adjusted with lime water;

[0042] (4) Use a transfer tube to transfer the mycelia transfer test tube formed in step (3) to a medium with a pH of 10.2, culture for 10 days, and keep the temperature at 25°C to form mycelia; the pH value can be adjusted with lime water.

[0043] (5) Use a transfer tube to transfer the hypha...

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Abstract

The invention relates to an acclimatization method for pleurotus ostreatus bacteria under a caustic environment. The method comprises the following steps that 1, the pH value of a culture medium wherethe pleurotus ostreatus bacteria are inoculated is adjusted within 6-6.5 through limewater, the culture medium is cultured for 10-15 days, and the temperature is kept within 25-28 DEG D; 2, hypha istransferred into a culture medium of which the pH value is larger than or equal to 7 and smaller than or equal to 8 and cultured for 10-15 days, and the temperature is kept within 25-28 DEG C; 3, thehypha is transferred into a culture medium of which the pH value is larger than or equal to 8 and smaller than or equal to 9 and cultured for 10-15 days, and the temperature is kept within 25-28 DEG C; 4 the hypha is transferred into a culture medium of which the pH value is larger than or equal to 9 and smaller than or equal to 11 and cultured for 10-15 days, and the temperature is kept within 25-28 DEG C; 5, the hypha is transferred into a culture medium of which the pH value is 11.5 and cultured for 10-15 days, and the temperature is kept within 25-28 DEG C. According to the acclimatizationmethod, acclimatized pleurotus ostreatus can adapt to the environment with high pH value, accordingly, growth of other bacteria is inhibited, and the yield of pleurotus ostreatus is increased.

Description

technical field [0001] The invention belongs to the technical field of oyster mushroom planting, in particular to a method for domesticating mushroom strains in a caustic environment. Background technique [0002] Pleurotus ostreatus cultivation has been successfully cultivated as early as the early 1980s, but during the rough decades, the development of the edible fungi industry has been slow. Most edible fungi survive in neutral medium, with a pH of about 6.5, but very miscellaneous fungi are suitable for survival in an acidic environment, which has a great impact on the yield and quality of edible fungi, especially oyster mushrooms. Contents of the invention [0003] The purpose of the present invention is to provide a method for domesticating Pleurotus ostreatus bacterial strains in a caustic environment, so that the Pleurotus ostreatus obtained after domestication can adapt to a high pH environment, thereby inhibiting the growth of miscellaneous bacteria and improving...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20C05G3/00
CPCC05B1/02C05G3/00C05C5/02C05D1/00C05D3/00C05D5/00C05F3/00C05F5/002C05F11/00
Inventor 张金霞黄晨阳肖俊培汪建党侯艳平史振霞闫训友侯晓强韩美玲王晶
Owner 翔天科技股份有限公司