A kind of continuous fermentation method of ethanol tolerance cellulase
A fermentation method and a cellulase endonuclease technology are applied in the field of continuous fermentation of ethanol-tolerant cellulase, and can solve the problems of decay and decline in enzyme production capacity.
Active Publication Date: 2020-05-26
中农华威生物制药(湖北)有限公司
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AI Technical Summary
Problems solved by technology
In existing studies, in order to induce Aspergillus niger to produce cellulase, the supply of carbon sources is often limited, so that the mycelium of Aspergillus niger gradually declines, and the ability to produce enzymes decreases
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0022] 1. Strains and materials
[0023] Aspergillus niger: Aspergillus niger ATCC 16888. Columnar activated carbon: Henan Runquan Purification Materials Co., Ltd. selects columnar activated carbon with a length of 2-3cm and a diameter of less than 1cm. Granular activated carbon: Gongyi Longda Water Treatment Materials Co., Ltd. selects granular activated carbon with a particle size of less than 2cm.
[0024] 2. Preparation of culture medium
[0025] PDA slant culture medium: Weigh 200 g of potatoes, peel and cut into pieces, add them to 1000 mL of tap water and boil for 20 min, then filter with gauze; add 20 g of glucose and 15 g of agar to the filtrate, sterilize at 115°C for 30 min, and invert the slant.
[0026] Fermentation medium: bran 30g / L, ammonium chloride 5g / L, potassium dihydrogen phosphate 2g / L, corncob flour 12g / L, peptone 0.01g / L, 5g / L glucose, natural pH, sterilization at 121°C 20min, filter with sterilized 6-layer gauze, and the filtrate is the fermentation...
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Abstract
The invention discloses a continuous fermentation method of ethanol-tolerance cellulase, and belongs to the field of enzyme engineering and fermentation engineering. The method provided by the invention comprises the following steps of spraying aspergillus niger spore liquid onto column activated carbon for culture so as to obtain activated column activated carbon; adding a culture medium and theactivated column activated carbon into a fermentation tank; performing fermentation for 1.5 to 3 days; adding active carbon nanometer powder and glucose; performing fermentation for 1.5 to 3d; then, adding active carbon nanometer powder; performing fermentation for 1 to 2 days; discharging fermentation mash out from the fermentation tank; performing filtering by a filter screen, wherein the filterliquid is first batch fermentation mash; the column active carbon for fixing the aspergillus niger hyphae on a filtering screen is used for next batch fermentation; repeating the fermentation steps for many times. The column activated carbon is used as a carrier of aspergillus niger hyphae; meanwhile, the feedback inhibition effect of the glucose is reduced by using the active carbon powder; thegoal of continuously generating ethanol-tolerance cellulase at high intensity can be reached.
Description
technical field [0001] The invention relates to the fields of enzyme engineering and fermentation engineering, in particular to a continuous fermentation method for ethanol-tolerant cellulase. Background technique [0002] Cellulase can degrade cellulose in grains and potatoes into fermentable sugars, and at the same time can promote amylase to degrade starch, thereby improving the yield of wine. The degradation of cellulose such as chaff or sorghum husk during the brewing process can improve the palatability of the distiller's grains feed and promote the high-quality development of the distiller's grains feed. Cellulase is of great significance to the production of "high quality, high yield and low consumption" in the wine industry. [0003] The world's annual photosynthesis produces more than 10 billion tons of cellulose resources. The use of green renewable cellulose resources to produce biofuels such as ethanol is of immeasurable significance for alleviating the greenh...
Claims
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IPC IPC(8): C12N9/42C12R1/685
CPCC12N9/2437C12Y302/01004
Inventor 薛栋升曾徐浩
Owner 中农华威生物制药(湖北)有限公司