Ratiometric Fluorescence Detection of Phosphate Based on Metal-Organic Framework Composites
A technology of metal-organic frameworks and composite materials, which is applied in the field of ratiometric fluorescence detection of phosphate based on metal-organic framework composites, which can solve the problems of time-consuming and cumbersome detection methods
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Embodiment 1
[0029] 1. Method for detecting phosphate based on ratiometric fluorescence of metal-organic framework composites
[0030] 1) Before the experiment, clean all the glassware, soak them in fresh aqua regia, wash them with ultrapure water, and dry them. UiO-66-NH used 2 Prepared by hydrothermal method, ZrCl 4 (169.06mg, 0.7264mmol) and diamino-tere-benzoic acid (131.2mg, 0.7264mmol) were dissolved in 80mL N,N-dimethylformamide (DMF), the solution was placed in a reaction kettle, and after 48 hours of reaction at 120°C, it was naturally cool down. Centrifugation and washing with DMF (3 times) gave a yellow powder. After drying under reduced pressure for 12 h, wash with dichloromethane three times, and dry under vacuum at 100 °C for 12 h to obtain UiO-66-NH 2 .
[0031] 2) Take 2mg of UiO-66-NH prepared in step 1) 2 Nanoparticles were dispersed in rhodamine B solution (4mL, 2mmol / L), ultrasonicated for 2h, centrifuged and washed twice with distilled water, dispersed with HEPES...
Embodiment 2
[0039] The method for detecting phosphate based on ratiometric fluorescence of metal-organic framework composites comprises the following steps:
[0040] 1) In a DMF solvent environment, add an equimolar amount of ZrCl 4 React with diamino-p-benzoic acid at 120°C for 48h, and cool naturally; centrifuge to collect the precipitate, wash with DMF for 3 times, dry under vacuum for 12h, wash with dichloromethane for 3 times, and then vacuum-dry at 100°C for 12h to obtain Metal Organic Framework UiO-66-NH 2 ;
[0041] 2) Take 2mg of the metal organic framework UiO-66-NH obtained in step 1) 2 , dispersed in 4 mL of rhodamine B solution with a concentration of 2 mmol / L, ultrasonically oscillated for 2 h, and the precipitate was collected by centrifugation, washed twice with distilled water, and the solid phase was dispersed in 4 mL of HEPES buffer with a concentration of 0.1 mol / L and a pH value of 7.4 solution, the metal-organic framework UiO-66-NH 2 / RhB;
[0042] 3) Take the m...
Embodiment 3
[0052] The method for detecting phosphate based on ratiometric fluorescence of metal-organic framework composites comprises the following steps:
[0053] 1) In a DMF solvent environment, add an equimolar amount of ZrCl 4 React with diamino-p-benzoic acid at 120°C for 48h, and cool naturally; centrifuge to collect the precipitate, wash with DMF for 3 times, dry under vacuum for 12h, wash with dichloromethane for 3 times, and then vacuum-dry at 100°C for 12h to obtain Metal Organic Framework UiO-66-NH 2 ;
[0054] 2) Take 2mg of the metal organic framework UiO-66-NH obtained in step 1) 2 , dispersed in 4 mL of rhodamine B solution with a concentration of 2 mmol / L, ultrasonically oscillated for 2 h, and the precipitate was collected by centrifugation, washed twice with distilled water, and the solid phase was dispersed in 4 mL of HEPES buffer with a concentration of 0.1 mol / L and a pH value of 7.4 solution, the metal-organic framework UiO-66-NH 2 / RhB;
[0055] 3) Take the m...
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