Method for quickly collecting cotton plant bug eggs

Abstract

The invention relates to a method for quickly collecting cotton plant bug eggs. The method comprises the steps that an appropriate quantity of adult cotton plant bugs are put in a transparent insect breeding box, wherein the ratio of the female insects to the male insects is close to 1:1; an opening of the insect breeding box is covered with nylon mesh cloth and sealed tightly, and absorbent cotton balls dipped with honey water are placed on the nylon mesh cloth; after the adult cotton plant bugs are supplemented with nutrients for at least 7 days, an appropriate amount of wet paper is placedin the insect breeding box and serves as a medium used for the oviposition of the cotton plant bugs; after the oviposition of the cotton plant bugs, the wet paper with the eggs is taken out of the insect breeding box, and therefore egg collection is completed. By means of the method, the eggs of apolygus lucorum can be quickly and easily collected, and the cost is saved; the cotton plant bugs oviposit on the surface of the paper, so that the quantity of the eggs is more conveniently counted, and the collected eggs can continue to be used for other tests.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for quickly and conveniently collecting Lygus larvae eggs. Background technique [0002] Due to the popularization and planting of transgenic cotton, important cotton pests such as cotton bollworm and pink bollworm have been effectively controlled, and the amount of pesticides used in cotton fields has been significantly reduced. However, the harm of minor pests such as the cotton bug has increased, and it has gradually risen to become the main pest of cotton fields. Therefore, it is of great significance to strengthen the research on the biology, ecology and outbreak mechanism of cotton Lygus pests for better formulation of control strategies. [0003] In the past, fresh green beans were mainly used to collect eggs. It was necessary to soak the green beans with 5% sodium hypochlorite to sterilize the green beans; Oviposition medium for stinkbug adults. After the eggs are...

AI Technical Summary

Problems solved by technology

Although the kidney beans have been sterilized, due to the long time of in vitro storage, rot and mildew often occur, resulting in a decrease in the hatching rate of Lygus cotton eggs; and the eggs in plant tissues are inconvenient to observe

In 2012, Chen Peiyu et al. reported "a method for collecting and hatching stinkbug eggs" (Plant Protection. 38(4): 105-107), which mainly uses 4 layers of wrinkled filter paper in a petri dish, and the central The method of humidifying the paper balls to collect the eggs of the green Lygus or the black Lygus, this method has problems such as: it is impossible to supplement the nutrients for the adults in the petri dish for a long time, and it is necessary to transfer the adults of the cotton Lygus with strong mobility when changing the filter paper.

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