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Preparation method of strain culture medium containing traditional Chinese medicinal materials

A strain culture medium and Chinese herbal medicine technology, applied in the field of microorganisms, can solve the problems of slow proliferation of Streptococcus pneumoniae and easy occurrence of bacterial contamination, and achieve the effects of reducing production costs, increasing production, and improving production efficiency

Pending Publication Date: 2018-05-08
CHENGDU OLYMVAX BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is that in the existing medium for cultivating Streptococcus pneumoniae, the proliferation rate of Streptococcus pneumoniae is relatively slow, and the phenomenon of bacterial contamination is prone to occur. The purpose is to provide a preparation method for a culture medium containing Chinese medicinal materials, Promotes a significant increase in the proliferation rate of Streptococcus pneumoniae, and is less prone to bacterial contamination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The present invention provides a method for preparing a culture medium containing Chinese medicinal materials, which includes the following steps:

[0024] Step 1. Weigh the agar, add water for injection to the agar and heat to 95°C to dissolve it, and dilute to 90% of the required volume after dissolution;

[0025] Step 2. Perform moist heat sterilization on the solution dissolved in step 1, at a temperature of 130°C, a pressure of 0.15 MPa, and a sterilization time of 20 minutes; cool the agar solution to 55-60°C for use;

[0026] Step 3. Weigh the following raw materials:

[0027] Carbon source: Jerusalem artichoke, 10g, Ganoderma lucidum, 10g, bagasse, 15g; Nitrogen source: cottonseed meal, 20g, peptone, 15g, shrimp skin powder, 20g, corn steep liquor, 15g, bran 10g; inorganic salts and trace elements : MgSO 4 , 0.20g / L; NaCl, 2.5g / L; K 2 HPO 4 , 0.1g / L; ZnSO 4 , 0.1g / L; FeSO 4 , 0.01g / L; Niacin: 0.2g / L; Adenine: 3.5g / L; Cysteine, 0.8g / L; Agar 12g; Water, 1000ml;

[0028] St...

Embodiment 2

[0035] The present invention provides a method for preparing a culture medium containing Chinese medicinal materials, which includes the following steps:

[0036] Step 1. Weigh the agar, add water for injection to the agar and heat to 95°C to dissolve it, and dilute to 90% of the required volume after dissolution;

[0037] Step 2. Perform moist heat sterilization on the solution dissolved in step 1, at a temperature of 130°C, a pressure of 0.15 MPa, and a sterilization time of 20 minutes; cool the agar solution to 55-60°C for use;

[0038] Step 3. Weigh the following raw materials:

[0039] Carbon source: Jerusalem artichoke, 35g, Ganoderma lucidum, 30g, bagasse, 40g; Nitrogen source: cottonseed meal, 80g, peptone, 45g, shrimp skin powder, 50g, corn steep liquor, 35g, bran, 30g; inorganic salt and trace amounts Element: MgSO 4 , 1.5g / L; NaCl, 12.5g / L; K 2 HPO 4 , 2.5g / L; ZnSO 4 , 0.2g / L; FeSO 4 , 0.12g / L; Niacin: 1.2g / L; Adenine: 8.5g / L; Cysteine, 2.0g / L; Agar 20g; Water, 1000ml;

[00...

Embodiment 3

[0047] The present invention provides a method for preparing a culture medium containing Chinese medicinal materials, which includes the following steps:

[0048] Step 1. Weigh the agar, add water for injection to the agar and heat to 95°C to dissolve it, and dilute to 90% of the required volume after dissolution;

[0049] Step 2. Perform moist heat sterilization on the solution dissolved in step 1, at a temperature of 130°C, a pressure of 0.15 MPa, and a sterilization time of 20 minutes; cool the agar solution to 55-60°C for use;

[0050] Step 3. Weigh the following raw materials:

[0051] Source: Jerusalem artichoke, 25g, Ganoderma lucidum, 20g, bagasse, 28g; Nitrogen source: cottonseed meal, 60g, peptone, 30g, shrimp skin powder, 40g, corn steep liquor, 25g, bran 20g; Inorganic salts and trace elements: MgSO 4 , 1.0g / L; NaCl, 6.5g / L; K 2 HPO 4 , 1.2g / L; ZnSO 4 , 0.16g / L; FeSO 4 , 0.08g / L; Niacin: 0.8g / L; Adenine: 6.0g / L; Cysteine, 1.6g / L; Agar 15g; Water, 1000ml;

[0052] Step 4. Us...

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Abstract

The invention discloses a preparation method of a strain culture medium containing traditional Chinese medicinal materials. The preparation method comprises the following steps: weighing agar, addinginjection water for dissolving, carrying out moist heat sterilization and cooling for later use; weighing the following raw materials: a carbon source is prepared from 10 to 35 parts of jerusalem artichoke, 10 to 30 parts of lucid ganoderma and 15 to 40 parts of bagasse; a nitrogen source is prepared from 20 to 80 parts of cottonseed meal powder, 15 to 45 parts of peptone, 20 to 50 parts of driedsmall shrimp powder, 15 to 35 parts of corn steep liquor and 10 to 30 parts of bran; inorganic salt and trace elements are as follows: 0.20 to 1.5g / L of MgSO4, 2.5 to 12.5 g / L of NaCl, 0.1 to 2.5 g / Lof K2HPO4, 0.1 to 0.2 g / L of ZnSO4, 0.01 to 0.12 g / L of FeSO4, 0.2 to 1.2 g / L of niacin, 3.5 to 8.5 g / L of adenine, 0.8 to 2.0 g / L of cysteine, 12 to 20g of agar and 1000 parts of water. By adopting the injection water to dissolve the weighed raw materials, determining the volume, adjusting a pH value, carrying out sterilization treatment, and then adding the treated material into a sterilized andcooled agar culture medium, a solid culture medium is prepared. The preparation method of the strain culture medium containing the traditional Chinese medicinal materials, disclosed by the invention,has the advantages that proliferation rate of streptococcus pneumoniae is significantly improved and the possibility of bacterial contamination is reduced.

Description

Technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for preparing a culture medium containing Chinese medicinal materials. Background technique [0002] Streptococcus pneumoniae was first isolated from patient sputum in France and the United States by Louis Pasteur and G.M. Sternberg in 1881. It is Gram-staining positive, the bacteria resemble spearheads, double or short-chained diplococci, virulent strains have capsules of polysaccharides in their body. 5% to 10% of normal people carry this bacteria in the upper respiratory tract, and virulent strains are important pathogens that cause human diseases. The strain has a certain application in the field of biotechnology, but the growth rate of Streptococcus pneumoniae in the commonly used culture medium is slow, and at the same time it is prone to infection. Summary of the invention [0003] The technical problem to be solved by the present invention is that in the exist...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/46
CPCC12N1/20
Inventor 张熠王文雷宇文彬
Owner CHENGDU OLYMVAX BIOPHARM