A carotenoid-containing composition and a preparation method of the carotenoid-containing composition
A carotene and composition technology, applied in the field of fermentation engineering, can solve the problems of unstable content and low yield, and achieve the effects of simple preparation method, high nutritional value and improved product quality
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[0022] Specifically, the preparation method of the composition may include, for example, the following steps: adding an activator during the fermentation of Mortierella alpina; after the fermentation, collecting the bacteria, separating and extracting oil to obtain a carotenoid-containing composition.
[0023] Alternatively, the Mortierella alpina in the embodiment of the present invention can be activated and cultivated through the following steps, for example: inoculate the Mortierella alpina species in the activation medium, and heat Cultivate under conditions for 46-50 hours to obtain seed activation culture solution. Wherein, the activation medium contains 1-2wt% of yeast powder and 4-8wt% of glucose.
[0024] The seed activation culture solution is inoculated into the expansion medium, and cultured at 26-30°C and 200-240r / min for 46-50h to obtain the seed expansion culture solution. Wherein, the expansion medium contains 1-2wt% of yeast powder and 4-8wt% of glucose.
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Embodiment 1
[0043] The Mortierella alpina strain was inoculated into the activation medium, and cultured at 26° C. and 200 r / min for 50 h to obtain a seed activation medium. The seed activation culture solution was inoculated into the expansion medium, and cultured at 26° C. and 200 r / min for 50 h to obtain the seed expansion medium. Wherein, both the activation medium and the expansion medium contain 1wt% of yeast powder and 4wt% of glucose, respectively.
[0044] According to the ratio of the inoculum size (volume ratio) of 18:100, the seed expansion culture solution was inoculated in a fermenter equipped with a fermentation medium for fermentation. The fermentation culture process is as follows: the culture temperature is 26°C, the stirring speed is 230r / min, the ventilation volume is 1.0vvm, the tank pressure is normal, and the culture time is 8 days. Wherein, the fermentation medium contains 1.5wt% of yeast powder, 6wt% of glucose, 0.05wt% of potassium dihydrogen phosphate and 0.005...
Embodiment 2
[0048] The Mortierella alpina strain was inoculated into the activation medium, and cultured at 30°C and 240r / min for 46 hours to obtain the seed activation medium. The seed activation culture solution was inoculated into the expansion medium, and cultured at 30° C. and 240 r / min for 46 hours to obtain the seed expansion medium. Wherein, the activation medium and the expansion medium both contain 2wt% of yeast powder and 8wt% of glucose.
[0049] According to the ratio of the inoculum amount (volume ratio) of 22:100, the seed expansion culture solution was inoculated in a fermenter equipped with a fermentation medium for fermentation. The fermentation culture process is as follows: the culture temperature is 30°C, the stirring speed is 270r / min, the ventilation volume is 1.4vvm, the tank pressure is normal, and the culture time is 8 days. Wherein, the fermentation medium contains 2.5wt% of yeast powder, 10wt% of glucose, 0.15wt% of potassium dihydrogen phosphate and 0.015wt% ...
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