Method for qualitatively and quantitatively analyzing non-volatile secondary metabolite chemical components of heartleaf houttuynia herb wall-broken medicinal slices

Abstract

The invention relates to a method for qualitatively and quantitatively analyzing non-volatile secondary metabolite chemical components of heartleaf houttuynia herb wall-broken medicinal slices. The method includes the steps: S1 pretreating the heartleaf houttuynia herb wall-broken medicinal slices to prepare sample solution; S2 taking a reference substance as a contrast, comparing pyrolysis fragmentation information with corresponding databases by the aid of a UHPLC-Q-TOF / MS (ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry) technique, and qualitativelyand quantitatively analyzing non-volatile secondary metabolite chemical components in a sample. A moving phase A is formic acid water solution, and a moving phase B is formic acid acetonitrile solution. According to the method, the non-volatile secondary metabolite chemical components of the heartleaf houttuynia herb wall-broken medicinal slices are qualitatively and quantitatively analyzed by theaid of the UHPLC-Q-TOF / MS technique, 36 non-volatile secondary metabolite products such as phenolic acids, flavonoid and alkaloids are detected, and the method has the advantages of high speed, highspecificity, low detection limit, high qualitative and quantitative ability and the like.

Description

technical field [0001] The invention relates to the technical field of detection of chemical components of traditional Chinese medicine preparations, in particular to a method for qualitative and quantitative analysis of chemical components of non-volatile secondary metabolites in broken-wall decoction pieces of Houttuynia cordata. Background technique [0002] Houttuynia cordata broken-wall decoction pieces is a broken-wall decoction piece product made from Houttuynia cordata as raw material by using traditional Chinese medicine wall-breaking technology. Houttuynia cordata is the dry aboveground part of Houttuynia cordata Thunb., a plant of the family Sanbaiaceae. It has the effects of clearing away heat and detoxification, eliminating carbuncle and discharging pus, diuresis and treating stranguria. Traditional Chinese medicine is mainly used for lung abscess and pus spit, phlegm heat, panting and coughing , heat dysentery, hot stranguria, carbuncle sore and other diseases....

AI Technical Summary

Problems solved by technology

However, since broken pieces of Chinese medicine do not have the morphological characteristics of traditional Chinese medicine pieces, breaking the wall will bring about changes in chemical components such as active ingredients or index components; therefore, component analysis of broken pieces of Chinese medicine is of great significance

[0003] The chemical components of Houttuynia cordata mainly include volatile oils, phenolic acids, flavonoids and alkaloids. At present, the volatile oil components are mainly detected by GC-MS, while the non-volatile secondary metabolites (phenolic acids, flavonoids and alkaloids, etc.), the existing technology is mainly focused on extraction, separation and identification, which cannot fully reflect the chemical substance basis of Houttuynia cordata, and it is difficult to reflect the diversity of active ingredients in Houttuynia cordata from different origins. Sexual difference and characteristic

Simultaneously in the prior art, the research on the chemical constituents of Houttuynia cordata broken wall decoction pieces has not been reported yet

Images