DNA-enzyme-producing strain- HL28-6 and application thereof
A DNase and strain technology, applied to DNase-producing strain HL28-6 and its application field, can solve the problem of difficult removal of odor, and achieve the effects of excellent characters, significant economic and social benefits, and high application potential
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Embodiment 1
[0023] The method for preparing DNase by the strain HL28-6 producing DNase comprises the following steps:
[0024] (1) Prepare LB slant medium: dissolve 10g of peptone, 5g of yeast powder, 10g of NaCl, add 300mL of water, adjust the pH to 6, add 20g of agar powder, add water to make up to 1000mL, heat and dissolve the agar, and put it in a container , sealed, sterilized at 121°C for 20 minutes, and placed on a slant to obtain LB slant medium;
[0025] (2) Preparation of fermentation medium: dissolve 10g of peptone, 5g of yeast powder, 10g of NaCl, add 300mL of water, adjust the pH to 6-8, add water to make up to 1000mL, mix well, put it in a container, seal it, and store it at 121°C Sterilize under high temperature for 20 minutes to obtain the fermentation medium;
[0026] (3) Prepare slant strains: use an inoculation loop to inoculate Bacillus HL28-6 strain on the slant medium prepared in step (1), and cultivate at a constant temperature of 25°C until the slant is covered wi...
Embodiment 2
[0030] The method for preparing DNase by producing DNase strain HL28-6 of the present invention comprises the following steps:
[0031] (1) Prepare LB slant medium: dissolve 10g of peptone, 5g of yeast powder, 10g of NaCl, add 300mL of water, adjust the pH to 7.2, add 20g of agar powder, add water to make up to 1000mL, heat and dissolve the agar, and put it in a container , sealed, sterilized at 121°C for 20 minutes, and placed on a slant to obtain LB slant medium;
[0032] (2) Preparation of fermentation medium: dissolve 10g of peptone, 5g of yeast powder, 10g of NaCl, add 300mL of water, adjust the pH to 6-8, add water to make up to 1000mL, mix well, put it in a container, seal it, and store it at 121°C Sterilize under high temperature for 20 minutes to obtain the fermentation medium;
[0033] (3) Prepare slant strains: use an inoculation loop to inoculate Bacillus HL28-6 bacterial strains on the slant medium prepared in step (1), and cultivate at a constant temperature of ...
Embodiment 3
[0037] The method for preparing DNase by the DNase-producing strain HL28-6 of the present invention comprises the following steps:
[0038] (1) Preparation of LB slant medium: Dissolve 10 g of peptone, 5 g of yeast powder, 10 g of NaCl, add 300 mL of water, adjust the pH to 9, add 20 g of agar powder, add water to make the volume to 1000 mL, dissolve the agar by heating, and place it in a container , sealed, sterilized at 121°C for 20min, placed on the slant to obtain LB slant medium;
[0039] (2) Preparation of fermentation medium: Dissolve 10 g of peptone, 5 g of yeast powder, and 10 g of NaCl, add 300 mL of water to dissolve, adjust the pH to 6-8, add water to dilute to 1000 mL, mix well, put in a container, seal, and store at 121° C. Under sterilization 20min, obtain fermentation medium;
[0040] (3) preparation of slanted surface strains: Bacillus HL28-6 bacterial strain is inoculated on the slanted surface medium prepared in step (1) with an inoculating loop, and cultiv...
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