Method for extracting cordyceps polysaccharide from solid culture medium residue of cordyceps militaris
A technology for solid culture medium and cordyceps polysaccharide, which is applied in the field of extracting cordyceps polysaccharide, can solve the problems of complicated operation, unsuitable for industrialized production, environmental pollution, etc., and achieves the effects of simple operation, reduced extraction cost, and edible safety.
Inactive Publication Date: 2018-05-29
辽宁省农业科学院大连生物技术研究所
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[0013] The above three methods all have the problems of complex operation and large amount of chemical reagents, which are time-consuming and laborious, pollute the environment, and have high extraction costs, so they are not suitable for industrial production.
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[0025] A method of extracting Cordyceps polysaccharides from Cordyceps militaris solid medium residues of the present invention is characterized in that following the steps in sequence:
[0026] a. Dry the residue of fresh Cordyceps militaris solid medium (wheat), take 10 kg and crush it into at least 60 mesh;
[0027] b. Add water according to the volume ratio of 1:10, homogenize with a homogenizer, and crush it with a high-pressure homogenizer to make a slurry;
[0028] c. Add cellulase with an enzyme activity of 10,000 U / g to 2% of the mass of the residue powder of the Cordyceps militaris solid medium, stir and enzymolyze at 50°C for 3 hours, and then add 2% of the mass of the raw material to an enzyme activity of 30,000 U / g U / g of pectinase, enzymatic hydrolysis at room temperature for 6 hours; this step is used to break the cell wall, so that the intracellular polysaccharide can be fully dissolved;
[0029] d. Warm up the enzymolysis solution to 65°C and stir for 2 hours...
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The invention discloses a method for extracting cordyceps polysaccharide from solid culture medium residue of cordyceps militaris, which orderly includes steps below: drying solid culture medium residue of fresh cordyceps militaris to be powder and then crushing to be slurry by a high-pressure homogenizer; adding cellulose according to 2% by mass of culture medium residue powder; stirring and performing enzymolysis for 3 hours at 50 DEG C, then adding pectinase according to 2% by mass of culture medium residue powder, and performing enzymolysis for 6 hours at room temperature; heating enzymatic hydrolysate to 65 DEG C and stirring for 2 hours; performing centrifugation at room temperature, and collecting supernatant; adding diastasum diastace according to 2% by mass of supernatant;, and stirring and performing enzymolysis for 6 hours at 55 DEG C; adding 1% of viscose of ZTC 1+1 nature clarifying agent B component according to 0.8% of solution volume; after stirring for 1 hour, centrifuging and collecting supernatant solution; performing alcohol precipitation and taking precipitation; after dissolving the precipitation, drying to be powder to obtain cordyceps polysaccharide.
Description
technical field [0001] The present invention relates to a method for extracting Cordyceps polysaccharides from Cordyceps militaris solid medium residues, in particular to a method for extracting Cordyceps polysaccharides from Cordyceps militaris solid medium residues with simple operation, high yield, high purity and no pollution . Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, Cordyceps militaris, etc., is a fungus of the genus Cordyceps, which can parasitize on the larvae or pupae of insects such as Lepidoptera, Coleoptera, and Diptera. Studies have shown that the types and contents of effective chemical components of Cordyceps militaris are the same or similar to those of Cordyceps sinensis, but they are different from the harsh growth conditions of Cordyceps sinensis, and have lower requirements on the growth environment, and artificial fermentation can be used for cultivation. The culture of Cordyceps militaris is divided into sol...
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CPCC08B37/0003
Inventor 孙永欣都兴范温志新孟楠李亚洁李学军米锐马淑慧
Owner 辽宁省农业科学院大连生物技术研究所