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HPLC determination method of oleanolic acid and ursolic acid in prunella vulgaris

A technology of oleanolic acid and ursolic acid, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of improved analysis efficiency, improved extraction efficiency, and simple preparation process

Inactive Publication Date: 2018-05-29
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Foreign scholars have applied this technology to the sample pretreatment in the quantitative and qualitative analysis of herbal medicines, but it is rarely used as a sample pretreatment method in the quantitative and qualitative analysis of active ingredients in Chinese medicinal materials.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Preparation of reference substance solution: Accurately weigh 54.2mg of oleanolic acid reference substance into a 50mL measuring bottle, add methanol to dissolve and set the volume to the mark, and make a reference substance solution with a mass concentration of 1.084mg / mL; Take 81.6mg of ursolic acid reference substance into a 100mL measuring bottle, add methanol to dissolve and make the volume to the mark, and make a reference substance solution with a mass concentration of 0.8160mg / mL.

[0021] (2) Prepare the sample solution to be tested: take the Prunella vulgaris medicinal material to be tested and grind it into powder, accurately weigh 0.5g of the sample powder, mix it with 1g of diatomaceous earth, put it in a 5mL extraction pool covered with a fiber filter membrane in advance, It is advisable to add diatomaceous earth to fill up the extraction tank, tighten the upper cover of the extraction tank, and perform rapid solvent extraction. The extraction solvent i...

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Abstract

The invention discloses an HPLC determination method of oleanolic acid and ursolic acid in prunella vulgaris, which includes the following steps: (1) preparing reference substance solutions: taking reference substances of the oleanolic acid and the ursolic acid, which are respectively added to methanol to make the reference substance solutions; (2) preparing a sample solution to be measured: taking the prunella vulgaris medicine and extracting in a rapid solvent, diluting the obtained extraction solution in the methanol to obtain the sample solution to be measured; (3) making standard curves:taking and mixing the oleanolic acidreference substance solutions and the ursolic acid reference substance solutions of different concentrations, and conducting quantitiative determination in a high performance liquid chromatograph to respectively obtain the standard curves of the oleanolic acid and the ursolic acid; (4) measuring the sample solution to be measured: taking the sample solution to be measured and conducting the quantitiative determination in the high performance liquid chromatograph to obtain peak areas of the oleanolic acid and the ursolic acid, and obtaining the contents of the oleanolic acid and the ursolic acid in the sample to be measured according to the standard curves of the oleanolic acid and the ursolic acid. The HPLC determination method of the oleanolic acid andthe ursolic acid in the prunella vulgaris has the advantages of being efficient in extraction and easy and fast in determination.

Description

technical field [0001] The invention relates to a method for detecting oleanolic acid and ursolic acid in Prunella vulgaris, in particular to a method for detecting oleanolic acid and ursolic acid in Prunella vulgaris by HPLC. Background technique [0002] Both oleanolic acid and ursolic acid are pentacyclic triterpenoids, which are active ingredients contained in various Chinese herbal medicines. Mediation and other pharmacological effects. The detection methods include spectrophotometry, thin-layer scanning and high-performance liquid chromatography. Oleanolic acid and ursolic acid are isomers of each other. Only the methyl positions at the 29 and 30 positions are different, and there are certain difficulties in separation. , To separate the two, the choice of chromatographic conditions is very important. In recent years, most of the methods used for HPLC determination have complex mobile phase compositions, long peak eluting time, and unsatisfactory peak shapes and resol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
Inventor 张云平陈启钊梁剑锋
Owner WUZHOU INST FOR FOOD & DRUG CONTROL