Reagent kit for determining serum cholyglycine and preparation method and use method of reagent kit

Abstract

The invention discloses a reagent kit of determining serum cholyglycine and relates to the technical field of medicine and biochemistry. The reagent kit comprises two independent liquid ingredients, namely a reagent R1 and a reagent R2, wherein the reagent R1 comprises a Tris buffer solution, PEG (polyethylene glycol)-8000, NaCl, tween-80 and glycerol, and a solvent is purified water; and the reagent R2 comprises a Tris buffer solution, tween-80, NaCl, trehalose, bovine serum albumin, a latex coated cholyglycine polyclonal antibody and NaN3, and a solvent is purified water. The invention further discloses a preparation method and a use method of the reagent kit for determining serum cholyglycine. Compared with the prior art, the reagent kit has the advantages that latex enhanced immunity turbidimetry for detecting cholyglycine in serum is a competition method, and the reagent kit for determining serum cholyglycine is simple to operate, non-pollution, high in sensitivity, good in specificity and low in cost.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring serum glycocholic acid suitable for automatic biochemical analysis and a preparation method thereof. Background technique [0002] Serum glycocholic acid (CG) is one of the conjugated bile acids in which bile acid and glycine are combined twice. In liver cells, cholesterol undergoes extremely complex enzymatic reactions and is converted into primary bile acids. Among them are cholic acid (CA) and chenodeoxycholic acid (CD-CA). There are three hydroxyl groups (C3, C7, C12) on the steroid core of cholic acid, and the hydroxyl group at the end of the side chain is combined with glycine through a peptide bond, with a molecular weight of 462U. [0003] The normal metabolic pathway of CG is the entero-hepatic circulation. CG is synthesized by liver cells, discharged into the gallbladder through the capillaries and bile ducts, and enters the duode...

AI Technical Summary

Problems solved by technology

[0007] However, radioimmunoassay (RIA) is polluted, has high requirements for environmental protection, and is difficult to dispose of radioactive waste; enzyme-linked immunosorbent assay (ELISA) is time-consuming and has poor quantitative results; homogeneous ELISA has poor anti-interference ability. Poor; more expensive equipment required for electrochemiluminescence

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