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A kind of glucagon protective agent and its application

A technology of glucagon and protective agent, which is applied in the field of polypeptide preservation to achieve the effects of reduced impact, stable performance, and simple preparation process

Active Publication Date: 2018-11-13
GUANGZHOU JINDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The present invention aims to solve the problems described above

Method used

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  • A kind of glucagon protective agent and its application
  • A kind of glucagon protective agent and its application
  • A kind of glucagon protective agent and its application

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Experimental program
Comparison scheme
Effect test

Embodiment

[0048] Protective agent preparation process:

[0049] 1. Prepare 100mL glycine-sodium hydroxide buffer solution with a concentration of 20-200mmol / L;

[0050] 2. Weigh curcumin, dipotassium EDTA, L-methionine, glutathione, and Tween-80 respectively, and dissolve them in glycine-sodium hydroxide buffer in turn; Take 0.01-1ml ProClin300, dissolve in the above solution; mix well, filter, and place at 2-8°C for later use.

[0051] 3. Take 10ml of the above solution, accurately weigh a predetermined amount of aprotinin, mix and dissolve; measure 2-400μl of DPP-IV inhibitor (teneliptin) with a concentration of 0.1mmol / mL, add the above solution, mix Mix well and store at 2-8°C.

[0052] The above materials are all commercially available; among them, aprotinin was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., curcumin was purchased from Sigma Company, and DPP-IV inhibitor (Teleliptin) was purchased from MedChemexpress Company.

[0053] Table 1 shows the specifi...

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Abstract

The invention discloses a glucagon protective agent, which comprises anticoagulant 10-100g / L; antioxidant 2.5-150g / L; serine protease inhibitor 1-100g / L; surfactant 0.1-5g / L; DPP‑IV inhibitor 0.02~4mmol / L; buffer solution 20~200mmol / L; biological preservative 0.1~10ml / L. The protective agent is mainly used for the protection of glucagon in blood samples, has stable performance, and has a significant effect on improving the stability of glucagon.

Description

technical field [0001] The invention relates to the field of polypeptide preservation, in particular to a glucagon protective agent and its application. Background technique [0002] Glucagon, also known as glucagon, is a hormone secreted by pancreatic islet α cells. It is a straight-chain polypeptide composed of 29 amino acids with a molecular weight of 3485. [0003] Diabetes mellitus is a syndrome of metabolic disorders characterized by chronically elevated blood sugar levels. Insulin and glucagon are important for maintaining proper blood sugar levels. Insulin acts on the liver and peripheral tissues by increasing peripheral uptake of glucose and reducing glucose output from the liver to lower blood glucose levels, while glucagon binds to glucagon receptors on liver cells, causing hepatic glycogen Breakdown releases glucose stored in the form of glycogen, which helps maintain glucose levels in the blood. As these stores are depleted, glucagon stimulates the liver to sy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 蔺涛李民友董超张玲罗宁齐宗献
Owner GUANGZHOU JINDE BIOTECH