Application of nanometer capsule body

A technology of thylakoids and nanometers, applied in the application field of nanothylakoids, can solve the problems of large side effects, poor biological safety, and complex preparation of nanomaterials, and achieve the effects of small side effects, low cost, and overcoming tumor hypoxia

Inactive Publication Date: 2018-06-22
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to overcome tumor hypoxia, improve the efficiency of tumor treatment, and solve the problems of complex preparation of existing anti-tumor nanomaterials, poor biological safety, and large side effe

Method used

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  • Application of nanometer capsule body
  • Application of nanometer capsule body
  • Application of nanometer capsule body

Examples

Experimental program
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Effect test

Example Embodiment

[0051] Example 1:

[0052] Step (1) Preparation of nano thylakoids:

[0053] The nano-thylakoid was prepared by osmotic lysis method. The specific procedure is as follows: Weigh 100g of fresh spinach leaves into a mortar and grind them into uniform fragments, then add 300 mL of a homogeneous buffer with a pH of 7.8 to the mortar.

[0054] After mixing, filter the filtrate with ten layers of gauze. Subsequently, the filtrate was collected, and the filtrate was centrifuged at 8000 rpm for 10 min to collect the precipitate to obtain the original chloroplast. The obtained original chloroplast was re-dispersed in the lysis buffer of HEPES (pH 8.0) with a concentration of 10mM and kept in the refrigerator at 4℃ for 2h, and then the lysed solution was centrifuged at 8000rpm for 10min to separate and remove the upper cytoplasm. Matrix fluid, collect the precipitate. Then the precipitate was washed twice with the above HEPES buffer and re-dispersed in secondary water at pH 7.4, and treate...

Example Embodiment

[0067] Example 2

[0068] Preparation of nano thylakoids:

[0069] The nano-thylakoid was prepared by osmotic lysis method. The specific method is as follows: Weigh 100 g of fresh spinach leaves into a mortar and grind them into uniform fragments, add 300 mL of a homogeneous buffer with pH 8.0 to the mortar, mix well, and filter with ten layers of gauze to collect the filtrate. Subsequently, the filtrate was collected, and the filtrate was centrifuged at 10000 rpm for 60 min to collect the precipitate to obtain the original chloroplast. The obtained original chloroplast was re-dispersed in 10mM HEPES (pH8.5) lysis buffer and kept in a 37℃ water bath for 5h, and then the lysed solution was centrifuged at 10000rpm for 60min to separate and remove the upper layer Cytoplasmic matrix fluid, collect the pellet. Then the precipitate was washed twice with the above HEPES buffer and re-dispersed in secondary water at pH 8.0, and treated with ultrasound in an ice bath (ultrasound frequenc...

Example Embodiment

[0071] Example 3

[0072] Preparation of nano thylakoids:

[0073] The nano-thylakoid was prepared by osmotic lysis method. The specific method is as follows: Weigh 100 g of fresh spinach leaves and place them in a mortar and grind them into uniform fragments, then add 300 mL of a homogeneous buffer with pH 6.0 to the mortar, mix well, and filter with ten layers of gauze to collect the filtrate. Subsequently, the filtrate was collected, and the filtrate was centrifuged at 4000 rpm for 5 min to collect the precipitate to obtain the original chloroplast. The obtained original chloroplast was re-dispersed in the lysis buffer of HEPES (pH 6.8) with a concentration of 10mM and kept in the refrigerator at 4℃ for 0.5h, and then the lysed solution was centrifuged at 4000rpm for 5min to separate and remove. The upper cytoplasmic matrix fluid, collect the precipitate. Then the precipitate was washed twice with the above HEPES buffer solution and re-dispersed in pH 6.0 secondary water, and...

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Abstract

The invention discloses application of a nanometer capsule body to preparation of anti-tumor medicines. Through the study, the inventor discovers that the nanometer capsule body can promote hydrogen peroxide accumulated in the tumor region to be decomposed into oxygen so that the tumor anoxia can be overcome; meanwhile, the light sensitive characteristic is realized, so that the nanometer capsulebody can be used as a light sensitive agent; the light power anti-tumor operation can be performed, so that the effect of combining the dual functions is achieved. Compared with a single PDT nanometermaterial, the nanometer capsule body has obviously improved PDT treatment effect.

Description

technical field [0001] The invention belongs to the field of biomedical materials; in particular, it relates to the application of a nano thylakoid. Background technique [0002] Cancer is the main killer of human death in the past decades, and the effectiveness of current cancer treatments is severely limited due to the complex tumor microenvironment. Among them, hypoxia is an inherent characteristic of solid tumors, which greatly increases the resistance of tumor therapy, making drug therapy and oxygen-dependent photodynamic therapy less effective. In addition, hydrogen peroxide (H 2 o 2 ) will accumulate in large quantities up to 100μM, while the concentration in normal tissues is only 20nM. Based on these special characteristics of the tumor region, many pharmaceutical agents that regulate the tumor microenvironment to achieve tumor therapeutic effects have been developed, but their biological safety and clinical effect are still open to question. In addition, photod...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K41/00A61K47/46A61P35/00
CPCA61K9/5176A61K41/0071
Inventor 刘又年欧阳江陈万松徐群芳
Owner CENT SOUTH UNIV
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