Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of a biological control agent in removing the pathogen of citrus sapling Huanglongbing

A biological control agent, technology for Huanglongbing, applied in the direction of chemicals for biological control, microorganism-based methods, applications, etc., can solve problems such as resurgence, inability to remove pathogens, and poisoning

Active Publication Date: 2021-08-17
HUAZHONG AGRI UNIV
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] First, use heavy pruning to restore the tree vigor of the diseased trees: carry out heavy pruning on the citrus trees, and keep the three major main branches and main side branches after short cutting. After two and a half years, strengthen the management of fertilizer and water and the management of diseases and insect pests , to restore the tree body so that it can bloom and bear fruit normally; but this method cannot remove the pathogen, and it will reappear in the later stage
[0010] Second, the pneumatic pressure injection system: the traditional injection method is still used in some places
However, the problem of root poisoning cannot be fundamentally solved, and it needs to be further improved with the treatment of the root system.
However, the problem of root poisoning cannot be fundamentally solved, and it needs to be further improved with the treatment of the root system.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of a biological control agent in removing the pathogen of citrus sapling Huanglongbing
  • Application of a biological control agent in removing the pathogen of citrus sapling Huanglongbing
  • Application of a biological control agent in removing the pathogen of citrus sapling Huanglongbing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The isolation and identification of embodiment 1 bacillus GJ1

[0034] 1. Isolation of bacteria

[0035] This strain was isolated from citrus leaves for the first time in the laboratory. These leaves were sterilized with 70% ethanol for 10 seconds, treated with 10% NaClO for 10 minutes and washed 5 times with sterile water, and then added PBS at a ratio of 1:100. (pH 7) buffer solution, inoculated in LB medium, cultivated at 37°C for 24-36h, isolated multiple bacterial strains, and carried out biological control tests on HLB by culturing and rooting seedlings for a single strain. Results GJ1 was confirmed to be effective for HLB control.

[0036] 2. Identification

[0037]Based on the 16S-23S rDNA interregional sequence (ITS), primers were designed for sequence amplification and sequencing. The measured sequence is shown in SEQ ID No.1. After sequencing and comparison of results, this study found that spores The 23S rDNA fragment sequencing of Bacillus GJ 1 has 99.99...

Embodiment 2

[0040] The application of embodiment 2 bacillus GJ1 in removing the huanglongbing pathogen of citrus saplings:

[0041] The technological process of Bacillus GJ1 to treat citrus Huanglongbing diseased young trees is as follows: figure 1 :

[0042] 1. Cultivation and inoculation of Bacillus spp. GJ1 in shake flasks: Prepare the medium according to the following medium components. After autoclaving, inoculate the activated Bacillus spp. 600 When it reaches 0.8-1.3, the bacillus GJ1 bacterial liquid is prepared, and the bacillus GJ1 bacterial liquid is used for seedling root irrigation treatment.

[0043] Table 1 Bacillus sp. GJ1 medium composition

[0044] Numbering Composition and content 1 Corn Flour 1% 2 Soy flour 1% 3 (NH 4 ) 2 SO 4 0.5%

4 K H 2 PO 4 0.01%

5 The balance is water pH 7.5

[0045] Two, citrus huanglongbing control treatment and detection method: adopt bacillus GJ1 bacterium liquid to remove th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses the application of a biological control agent in removing the pathogen of Huanglongbing of citrus saplings. The biological control agent includes Bacillus GJ1, which was deposited in China Center for Type Culture Collection on July 28, 2017, with the preservation number CCTCC M 2017456. The application comprises the following steps: using the above-mentioned bacillus GJ1 bacterial solution to remove the seedlings infected by Huanglongbing. The invention shows that the bacillus GJ1 bacterial agent has a certain detoxification effect on Huanglongbing, and the purpose of removing the pathogen can be achieved through repeated inoculation until the seventh treatment.

Description

technical field [0001] The invention belongs to the technical field of biological control agents, and in particular relates to the application of a biological control agent in removing the pathogen of citrus sapling Huanglongbing. Background technique [0002] Bacillus sp. belongs to the genus Bacillus. During the growth process, it can produce a series of metabolites that can inhibit the activity of fungi and bacteria, and has a strong inhibitory effect on plant pathogenic bacteria. The bacterium is widely distributed in nature, easy to isolate and cultivate, non-toxic and harmless to humans and animals, and does not pollute the environment; its metabolites are relatively rich, have broad-spectrum antibacterial activity and strong stress resistance, fast growth, and good stability. [0003] Bacillus is a bacterium with broad-spectrum antibacterial activity, strong ability to produce secondary metabolites, and can produce a variety of antibacterial substances. The research ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 姜玲赵秀云丁圆席姜乾坤孙亮南京
Owner HUAZHONG AGRI UNIV