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High-frequency somatic embryo regeneration culture medium without germplasm gene type limitation and application thereof

A technology for regeneration medium and culture medium, applied in application, plant regeneration, horticulture, etc., can solve the problems such as genotype limitation of Zoysias germplasm, improve the frequency of somatic embryo generation and regeneration plant frequency, overcome Different cultivars and genotype barriers, the effect of increasing the frequency of regenerated plants

Active Publication Date: 2018-07-06
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the above studies can neither solve the problem of genotype restriction of Zoysias germplasm, nor involve the problem of high-frequency somatic embryo regeneration of different Zoysias germplasm genotypes. The bottleneck of rapid multiplication

Method used

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  • High-frequency somatic embryo regeneration culture medium without germplasm gene type limitation and application thereof
  • High-frequency somatic embryo regeneration culture medium without germplasm gene type limitation and application thereof
  • High-frequency somatic embryo regeneration culture medium without germplasm gene type limitation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Somatic Embryo Induction Medium

[0038] Somatic embryo induction medium: improved MS+2mg / L 2,4-D+0.2mg / L NAA+0.1mg / L 6-BA+40g / L sucrose+0.7% agar, pH 5.8.

[0039] This modified MS medium includes macronutrients, micronutrients and organic reagents:

[0040] The components of macronutrients and their corresponding concentrations are as follows:

[0041]

[0042] The components of micronutrients and their corresponding concentrations are as follows:

[0043]

[0044] The components of organic reagents and their corresponding concentrations are as follows:

[0045]

Embodiment 2

[0046] Example 2 Somatic Embryo Induction Medium

[0047] Basically the same as Example 1, the difference is that the somatic embryo induction medium is improved MS+4mg / L 2,4-D+0.2mg / L NAA+0.1mg / L 6-BA+40g / L sucrose +0.7% agar, pH 5.8.

Embodiment 3

[0048] Example 3 Somatic Embryo Induction Medium

[0049] Basically the same as Example 1, the difference is that the somatic embryo induction medium is improved MS+2mg / L 2,4-D+0.1mg / L NAA+0.05mg / L 6-BA+50g / L sucrose +0.6% agar, pH 6.0.

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Abstract

The invention discloses a somatic embryo induction culture medium. The somatic embryo induction culture medium is prepared from an improved MS (Murashige-Skoog) culture medium, 2 to 4mg / L of 2,4-D, 0.2mg / L of NAA (Naphthylacetic Acid), 0.1mg / L of 6-BA, 40g / L of sucrose and 0.7 percent of agar, and has the pH (Potential of Hydrogen) value of 5.8 to 6.0. The invention further discloses an embryoid proliferation growth combined culture medium and a high-frequency somatic embryo regeneration culture medium without germplasm gene type limitation. The invention further discloses application of the somatic embryo induction culture medium and the combined culture medium. According to the somatic embryo induction culture medium disclosed by the invention, suitable explants are selected, and a culture medium formula, hormone component concentration, corresponding culture conditions and the like are adjusted in time according to a key development phase of the explants, so that the factors reach abetter cooperative interaction effect; different types (verities) and gene type limitation problems and the high-frequency somatic embryo regeneration problem in a zoysia japonica culture process areeffectively overcome, and the somatic embryo generation frequency and the regeneration plant frequency are greatly improved.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a high-frequency somatic embryo regeneration medium without germplasm genotype restriction and application thereof. Background technique [0002] Plants of the genus Zoysia (Zoysia genus) are mainly distributed in the vast area of ​​eastern Asia, spanning about 20 latitudes from north to south, and 30 longitudes from east to west; in my country, the main distribution area is the coastal strip from Liaoning in the northeast to Guangxi in the south. Due to the complex and diverse ecological environment of Zoysia grass, its typical and strict cross-pollination characteristics have led to the existence of a large number of interspecific hybrids and natural hybrids of Zoysia grass, and the large intra-specific variation also makes natural wild populations maintain rich natural variation among individuals. , genotype differences and genetic diversity. [0003] Zoysia has become a high-qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 梁慧敏梁栋刘艳韩鹏胡德龙周兴元夏阳吴璇
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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