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A kind of stevia directional hybrid seed production method

A technology of hybrid seed production and stevia, applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problems of difficult selection in the later stage, large waste of male plants, low seed harvest rate, etc., and achieve improved stability and controllability, lower production costs, and mitigate the effects of complex components

Active Publication Date: 2021-03-05
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have the following disadvantages: there are high flowering requirements between the male parent and the female parent, low hybrid seed setting rate, low seed purity, large waste of male parent plants, and high seed production costs
The purity of the seeds obtained by the former seed harvesting method is relatively high, but the disadvantages are low seed harvest rate, inconsistent maturity, low germination rate, and difficult selection in the later stage; the seed maturity consistency obtained by the latter harvest method is better, and the seeds The yield is high, but the obtained seeds have low purity and high degree of mixing, which is labor-intensive and time-consuming. At the same time, it is easy to cause damage to the plants, resulting in the later harvested seeds containing immature seeds falling off the injured branches
For this reason, these two harvesting methods ultimately lead to the disadvantages of low purity of hybrid seeds, low germination rate, high production cost and low field planting efficiency in the later stage.
[0006] In addition, in the preparation of artificial pollination solutions for other crops that have been published so far, the required components are complex. For example, the pollination solution formula disclosed in the patent 201610497946.2 contains sucrose, glucose, boric acid, guar gum, soybean lecithin, lauryl Ten compounds such as hydroxypropyl phosphate, betaine, potassium nitrate, ammonium chloride, and choline chloride. The increase in components makes it difficult for ordinary farmers to purchase in sufficient quantities and make accurate preparations, which affects the success rate of pollination and the effective promotion of technologies.

Method used

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  • A kind of stevia directional hybrid seed production method
  • A kind of stevia directional hybrid seed production method
  • A kind of stevia directional hybrid seed production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] A stevia directional hybrid seed production method, comprising the steps of:

[0067] The male parent material used was Nannong 1066 cutting propagation seedling, and the female parent material was Tian Nong No. 1 cutting propagation seedling.

[0068] (1) Collection of small flowers: After Nannong 1066 plants enter the flowering period, before 10:00 in the morning, pick and fix the unopened small flowers with liquid nitrogen and store them at -80°C for later use;

[0069] (2) Preparation of pollen suspension buffer: Weigh the following substances, 10 parts of D-sodium erythorbate, 50 parts of polyethylene glycol 4000, and 10 times the total mass of both deionized water, to obtain pollen suspension buffer for use.

[0070] (3) Preparation of pollination solution: take the florets preserved in step (1), freeze, grind and pulverize, add pollen suspension buffer at a weight ratio of 1:50, homogenize under ice bath conditions, filter through four layers of gauze, and pass t...

Embodiment 2

[0087] A stevia directional hybrid seed production method, comprising the steps of:

[0088] The male parent material used was Nannong 1066 cutting propagation seedling, and the female parent material was Tian Nong No. 1 cutting propagation seedling.

[0089] (1) Collection of small flowers: After Nannong 1066 plants enter the flowering period, before 10:00 in the morning, pick and fix the unopened small flowers with liquid nitrogen and store them at -120°C for later use;

[0090] (2) Preparation of pollen suspension buffer: Weigh the following substances, 1 part of D-sodium erythorbate, 50 parts of polyethylene glycol 4000, and 15 times the total mass of the two deionized water, to obtain a pollen suspension buffer for use.

[0091] (3) Preparation of pollination solution: take the florets preserved in step (1), freeze, grind and pulverize, add pollen suspension buffer at a weight ratio of 1:50, homogenize under ice bath conditions, filter through four layers of gauze, and pa...

Embodiment 3

[0108] A stevia directional hybrid seed production method, comprising the steps of:

[0109] The male parent material used was Nannong 1066 cutting propagation seedling, and the female parent material was Tian Nong No. 1 cutting propagation seedling.

[0110] (1) Collection of small flowers: After Nannong 1066 plants enter the flowering period, before 10:00 in the morning, pick and fix the unopened small flowers with liquid nitrogen and store them at -200°C for later use;

[0111] (2) Preparation of pollen suspension buffer: Weigh the following substances, 10 parts of D-sodium erythorbate, 1 part of polyethylene glycol 4000, and 20 times the total mass of both deionized water, to obtain pollen suspension buffer for use.

[0112] (3) Preparation of pollination solution: take the florets preserved in step (1), freeze, grind and pulverize, add pollen suspension buffer at a weight ratio of 1:50, homogenize under ice bath conditions, filter through four layers of gauze, and pass thro...

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Abstract

The invention discloses a method for producing stevia directional hybridization, which belongs to the field of economic crop seed production. The method includes isolated planting of parental plants, collection and preservation of pollen, preparation of pollination solution, liquid spray or liquid dripping pollination and collection of stevia seeds. According to the stevia directional hybrid seed production method of the present invention, the hybrid seed setting rate can be improved, the hybrid seed purity can be guaranteed, and the seed harvest rate can be improved.

Description

technical field [0001] The invention belongs to the field of economic crop seed production, and in particular relates to a stevia directional hybrid seed production method. Background technique [0002] Stevia is a self-incompatibility plant. Most varieties are self-pollinating and do not bear fruit. They rely on hybridization to produce seeds. [0003] The existing stevia hybrid seed production method is to plant the cutting propagating seedlings or direct-seeding seedlings of each parental strain in the same field, and use honeybees and other media to pollinate and cross each other to prepare stevia hybrid seeds. These methods have the following disadvantages: there are high requirements for flowering encounter between the male parent and the female parent, low hybrid seed setting rate, low seed purity, large waste of male parent plants, and high seed production costs. [0004] In addition, in the existing stevia hybrid seed production method, different parents are plante...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 罗庆云袁鎏柳杨凯祺
Owner NANJING AGRICULTURAL UNIVERSITY