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A kind of bone-specific calcium fluorescence staining method and its application

A fluorescent dyeing and specific technology, applied in the field of biotechnology, which can solve the problems of time-consuming, complicated steps, and the inability to observe the bone quality change process in real time in vivo

Active Publication Date: 2022-01-18
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] It can be seen that the traditional alizarin red calcium staining method is complicated and time-consuming. Before staining, the specimen needs to be fixed, and it is impossible to observe the change process of bone quality in real time.

Method used

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  • A kind of bone-specific calcium fluorescence staining method and its application
  • A kind of bone-specific calcium fluorescence staining method and its application

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Embodiment 1

[0040] Example 1 Calcium-specific fluorescent staining of wild-type zebrafish cartilage

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Abstract

The invention belongs to the field of biological technology, and relates to a bone-specific calcium fluorescent staining method and its application. The method uses natural extracts of purpurin, lucidin or 3-hydroxybaciquinone (3-hydroxyl hydroxy‑morindone) as a fluorescent stain for fluorescently labeling teleosts in living vertebrates such as zebrafish and mice. The method is simple and quick to operate, and can be used for dynamic observation of living body, and the hard bone can be clearly observed by fluorescent staining. This method provides a new staining protocol and tool for the examination of cartilage and hyperplastic bone tissue in clinical samples.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for fluorescent staining of cartilage-specific calcium and its application, in particular to a method for fluorescent staining that can specifically mark the cartilage of living vertebrates and its application. Purpurin, lucidin, or 3-hydroxy-morindone are used as fluorescent stains to fluorescently label teleosts in living vertebrates such as zebrafish and mice. This method can be used for live dynamic observation. Background technique [0002] The prior art discloses that purpurin (CAS: 81-54-9), also known as 1,2,4-trihydroxyanthraquinone, urorubin, purpurin, viologen, is a natural component of madder, As a red / yellow dye, it can be used for fast dyeing of cotton cloth. Studies have shown that purpurin can form complexes with a variety of metal ions, and can be used as reagents for detecting zirconium, boron, and calcium; in addition, studies have also shown that ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 王旭孙少阳党永军
Owner FUDAN UNIV