Application of sanguinarine in preparation of medicine for treating animal trichinosis
A technology of sanguinarine and trichinella, applied in the field of medicine, can solve problems such as side effects of anti-trichinella drugs, achieve the effect of reducing pathological damage, significant effect, and strong killing effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0017] Example 1 Preparation of sanguinarine for treating animal trichinosis
[0018] 1 Extraction method of sanguinarine
[0019] Take 6 kg of small fruit powder, add 75% ethanol, reflux on a water bath at 65°C for 30 minutes, evaporate in vacuo at 70°C, resuspend in distilled water, and use petroleum ether (boiling range 30-60°C), chloroform, and ethyl acetate in sequence. Esters, n-butanol, and distilled water were extracted under hot reflux in a water bath. After concentration, use silica gel column chromatography, use petroleum ether-ethyl acetate solvent system as eluent, and use the method of gradient elution to carry out column chromatography separation on the petroleum ether extract obtained by solvent treatment, and check by TLC. Those with the same or similar components were combined to obtain six compounds of A, B, C, D, E, and F. Section D is separated by secondary and tertiary column chromatography to finally obtain four relatively pure compounds, numbered Ⅰ, Ⅱ...
Embodiment 2
[0032] Example 2 In vitro insecticidal experiment of sanguinarine against Trichinella spiralis
[0033] 1. Isolation of Trichinella spiralis
[0034] 1) Isolation of Trichinella spiralis muscle larvae
[0035] BALB / c mice were orally infected with 300 Trichinella spiralis muscle larvae. After 40-60 days, the mice were killed by cervical vertebrae breaking, the fur and viscera were removed, the muscles were minced, put into a beaker, and 1% HCI and 1% stomach were added. Artificial digestive juice digestion with protease (the ratio of muscle to digestive juice is 1:7), stirred with a glass rod for 30 min, placed in a 37°C incubator for 16 h, stirring occasionally, after the meat dregs were completely digested, use 60 min Remove the residue with a mesh filter, wash the filtrate with 0.9% normal saline at 37°C, pour off the supernatant after the worms have settled for 15-20 minutes, add an appropriate amount of normal saline to precipitate and wash, repeat 3 times, and finally s...
Embodiment 3
[0049] Example 3 In vivo insecticidal experiment of sanguinarine against Trichinella spiralis
[0050] 6-week-old female BALB / C mice, weighing 18-20 g, were purchased from Beijing Huafukang Biotechnology Co., Ltd.
[0051] The Trichinella spiralis of the female Kunming rat species was preserved by the Animal Parasite Laboratory of the College of Animal Science and Technology, Jilin Agricultural University.
[0052] 1. In vivo experiment of sanguinarine against adult Trichinella spiralis
[0053] 60 6-week-old female BALB / C mice, 10 were randomly selected as blank control, and the remaining 50 were each infected with 300 Trichinella spiralis muscle larvae, and were randomly divided into 5 groups (challenge group, DMSO control group, Albenda azole group, 70ppm group, 80ppm group). 24 hours after infection, mice in each group were orally administered normal saline, DMSO, 50 mg / kg albendazole, 70 ppm and 80 ppm sanguinarine, respectively. The mice were killed on the 7th day aft...
PUM
| Property | Measurement | Unit |
|---|---|---|
| absorbance | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More