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Protein chip for detecting clinical common pathogenic microorganisms, and preparation method thereof

A pathogenic microorganism and protein chip technology, which is applied in the field of protein chips for detecting common clinical pathogenic microorganisms and their preparation, and achieves the effects of good performance, highly consistent detection rate and convenient detection.

Inactive Publication Date: 2018-08-10
江苏三联生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention can effectively overcome the problem that the traditional identification method cannot do anything for microorganisms that cannot be cultured or need to be cultured in a long room and under special conditions, and also realizes the fully automatic operation of pathogenic microorganism identification

Method used

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  • Protein chip for detecting clinical common pathogenic microorganisms, and preparation method thereof
  • Protein chip for detecting clinical common pathogenic microorganisms, and preparation method thereof
  • Protein chip for detecting clinical common pathogenic microorganisms, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A protein chip for detecting common clinical pathogenic microorganisms, the protein chip is prepared through the following steps:

[0029] (1) Pretreatment of black glass slide;

[0030] ① Soak the black glass slide in the glass slide pretreatment solution containing NaOH for 16 hours, and then wash it with purified water for 2 to 8 times;

[0031] ②Soak the black glass slide in a silane aqueous solution with a mass concentration of 1 wt% for 20 minutes;

[0032] ③Put the soaked black glass slides into the oven and bake at 180℃ for 0.2h.

[0033] (2) Spot antibody solution;

[0034] Reference figure 1 , Using automatic machine spotting, spot on the black glass slide into a 4×14 matrix; each column is a spot formed by the antibody solution of the same strain; the antibody solution includes E. coli, Staphylococcus aureus, Salmonella, and Pseudomonas , Vibrio cholerae, Klebsiella, Listeria, Streptococcus, Shigella, Adenovirus, Acinetobacter baumannii, Staphylococcus haemolyticus.

...

Embodiment 2

[0040] A protein chip for detecting common clinical pathogenic microorganisms, the protein chip is prepared through the following steps:

[0041] (1) Pretreatment of black glass slide;

[0042] ①Soak the black glass slide in the glass slide pretreatment solution containing NaOH for 24 hours, and then wash it with purified water for 2-8 times;

[0043] ②Soak the black glass slide in a silane aqueous solution with a mass concentration of 0.05wt% for 60 minutes;

[0044] ③Put the soaked black glass slide in the oven and bake it at 140℃ for 0.4h.

[0045] (2) Spot antibody solution;

[0046] Reference figure 1 , Using automatic machine spotting, spot on the black glass slide into a 4×14 matrix; each column is a spot formed by the antibody solution of the same strain; the antibody solution includes E. coli, Staphylococcus aureus, Salmonella, and Pseudomonas , Vibrio cholerae, Klebsiella, Listeria, Streptococcus, Shigella, Adenovirus, Acinetobacter baumannii, Staphylococcus haemolyticus.

[0...

Embodiment 3

[0052] A protein chip for detecting common clinical pathogenic microorganisms, the protein chip is prepared through the following steps:

[0053] (1) Pretreatment of black glass slide;

[0054] ①Soak the black glass slide in the glass slide pretreatment solution containing NaOH for 20 hours, and then wash it with purified water for 2-8 times;

[0055] ②Soak the black glass slide in a silane aqueous solution with a mass concentration of 0.5 wt% for 30 minutes;

[0056] ③Put the soaked black glass slide into the oven and bake it at 100℃ for 0.6h.

[0057] (2) Spot antibody solution;

[0058] Reference figure 1 , Using automatic machine spotting, spot on the black glass slide into a 4×14 matrix; each column is a spot formed by the antibody solution of the same strain; the antibody solution includes E. coli, Staphylococcus aureus, Salmonella, and Pseudomonas , Vibrio cholerae, Klebsiella, Listeria, Streptococcus, Shigella, Adenovirus, Acinetobacter baumannii, Staphylococcus haemolyticus.

...

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Abstract

The invention discloses a protein chip for detecting clinical common pathogenic microorganisms. The protein chip is prepared by the following steps: (1) black slide pretreatment; (2) sample application of an antibody solution; (3) blocking of the antibody solution to prepare the protein chip. The protein chip is used for detecting the pathogenic microorganisms, has a high detection rate, and alsorealizes fully-automatic operation of identification of the pathogenic microorganisms.

Description

Technical field [0001] The invention relates to the technical field of microbial detection, in particular to a protein chip for detecting common clinical pathogenic microorganisms and a preparation method thereof. Background technique [0002] In the 1970s, the development of computer and digital information technology combined with microbial technology gave birth to the microbial coding identification technology, and then created semi-automatic and fully automatic microbial identification and drug sensitivity analyzers, making microbiology from the traditional manual operation technology Entered the era of automation and information. [0003] Since the 1990s, the rapid development of immunology technology is penetrating into the field of microbes, and various immunolabeling and analysis technologies have been developed from traditional color development, fluorescence and radioimmunoassay to inter-chamber distinguished fluorescence, chemiluminescence and other technologies. Greatl...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/535G01N21/76
CPCG01N21/763G01N33/535G01N33/569G01N2333/005G01N2333/195Y02A50/30
Inventor 成舜施启尧丁俊杰泮锋纲
Owner 江苏三联生物工程股份有限公司
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