Microbial agent for relieving continuous cropping obstacle of melon crop and application of microbial agent

A technology for microbial inoculants and continuous cropping obstacles, which is applied in the field of microorganisms and continuous cropping obstacle restoration, can solve problems such as restricting the sustainable development of melon crop management and the melon crop industry, and achieves the improvement of soil physical and chemical properties, simple preparation method and reasonable formula. Effect

Inactive Publication Date: 2018-08-17
SHENYANG AGRI UNIV
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AI Technical Summary

Problems solved by technology

At present, there are relatively few probiotics used in the treatment of continuous cropping obstacles and autotoxicity of melon crops, which seriously restricts the control of continuous cropping obstacles of melon crops and the sustainable development of the melon crop industry.

Method used

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  • Microbial agent for relieving continuous cropping obstacle of melon crop and application of microbial agent
  • Microbial agent for relieving continuous cropping obstacle of melon crop and application of microbial agent
  • Microbial agent for relieving continuous cropping obstacle of melon crop and application of microbial agent

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Embodiment Construction

[0023] 1. Materials and Methods

[0024] 1.1 The medium used to isolate Burkholderia is an inorganic salt medium, and the formula includes MgSO 4 ·7H 2 O0.2g / L, CaCl 2 2H 2 O 0.02g / L, FeCl 3 0.05g / L, KH 2 PO 4 1.0g / L, K 2 HPO 4 1.0g / L, NH 4 NO 3 1.0g / L, cholesterol 0.3g / L, and agar 17g / L respectively with a treatment concentration of 200μg·mL -1 The p-hydroxybenzoic acid, benzoic acid and cinnamic acid are used as the sole carbon source, and the pH value is adjusted to neutral.

[0025] 1.2 The method used to isolate Burkholderia:

[0026] Weigh 10g of melon crop rhizosphere soil and grind it into a 250mL Erlenmeyer flask, add 90mL of sterile water to shake well, and shake it on a shaker at 120r min -1 Shake for 30 minutes to fully mix the soil sample and make 10 -1 double dilution; use a pipette to draw 1mL10 -1 Transfer the diluted solution into a sterilized test tube filled with 9mL sterile water, and make 10 -2 double dilution; then use a pipette to draw ...

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Abstract

The invention relates to a microbial agent for relieving a continuous cropping obstacle of a melon crop and application of the microbial agent. The microbial agent is classified and named as Burkholderia phytofirmans, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), of which the address is Institute of Microbiology Chinese Academy of Science, No.3, yard 1,Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No. 15326, the preservation date is January 29th, 2018, and the Burkholderia phytofirmans can be used for preparing themicrobial agent. The microbial agent has good rhizosphere colonization capacity for a melon crop plant, is safe and reliable, has good degradation effect on autotoxins in continuous cropped melon cropsoil, improves the physical and chemical properties of soil remarkably, and meanwhile, has a remarkable promotional effect on stress resistance of the melon crop plant, and the microbial agent is simple in preparation method and reasonable in formula, and can be used for control of the continuous cropping obstacle of the melon crop.

Description

technical field [0001] The invention relates to the field of repairing microorganisms and continuous cropping obstacles, in particular to a microbial agent for alleviating continuous cropping obstacles of melon crops and an application thereof. Background technique [0002] Melon crops contain various components necessary for human nutrition, such as protein, fat, carbohydrates, Ca, P, Fe, carotene, and vitamins. Many documents and physical research have found that China has a history of more than 3,000 years of cultivating melon crops, and is one of the earliest countries in the world to cultivate melon crops. [0003] In recent years, melon farmers have blindly pursued high yields and excessively applied chemical fertilizers and pesticides, resulting in the destruction of the soil ecological environment and excessive pesticide residues. Population increase and urban development have led to a gradual decrease in the area of ​​cultivated land. As a result, facility agricult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/00A01P21/00C12R1/01C09K101/00
CPCA01N63/00C09K17/14C09K2101/00C12N1/205C12R2001/01
Inventor 高增贵张一迪马慧姚远刘限
Owner SHENYANG AGRI UNIV
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