Method and device for automatically sorting and labeling bacteria on basis of immune processes

Abstract

The invention discloses a method and a device for automatically sorting and labeling bacteria on the basis of immune processes. The device comprises a tank, feet, a liquid outlet pipe and stirring rollers. The feet are arranged on the periphery of the bottom surface of the tank, the liquid outlet pipe is arranged beside the feet, an end cover is arranged at an end of the liquid outlet pipe, a motor base is arranged on the top surface of the tank, and a servo motor is arranged on the motor base. The method and the device have the advantages that the input quantities of antibody molecules in storage bins can reach preset values under the automatic control of a feedback control circuit which comprises flow sensors, a control host and electromagnetic valves, the stirring rollers can be drivenby the servo motor to rotate, accordingly, to-be-detected sample liquid and the antibody molecules can be stirred, and the bacterium sorting and labeling work efficiency can be improved; video information of bacterium sorting and labeling work states can be acquired by an infrared camera in real time and can be displayed on a display screen in real time, accordingly, users can conveniently accurately master the bacterium sorting and labeling work progress, and the method and the device are favorable for wide-range popularization.

Description

technical field [0001] The invention relates to the technical field of biological sample sorting, in particular to a method and device for automatically sorting and marking bacteria based on an immune method. Background technique [0002] With the continuous development of life sciences, the basic research of bacterial biology and immunology, as well as the diagnosis and treatment of clinical diseases require the separation and purification of bacteria from mixed samples containing different bacteria. The existing bacterial separation technology mainly includes Ficoll density gradient centrifugation. , centrifugal elution, two-phase separation, etc. These methods are separated by physical properties such as bacterial size, density, and surface charge, and it is difficult to obtain ideal purity and recovery. Cell separation based on magnetic beads A cell separation technology developed rapidly in the past, firstly label antibody molecules on the magnetic beads to make them sp...

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Problems solved by technology

[0002] With the continuous development of life sciences, the basic research of bacterial biology and immunology, as well as the diagnosis and treatment of clinical diseases require the separation and purification of bacteria from mixed samples containing different bacteria. The existing bacterial separation technology mainly includes Ficoll density gradient centrifugation. , centrifugal elution, two-phase separation, etc. These methods are separated by physical properties such as bacterial size, density, and surface charge, and it is difficult to obtain ideal purity and recovery. Cell separation based on magnetic beads A cell separation technology developed rapidly in the past, firstly label antibody molecules on the magnetic beads to make them specifically bind to the cells, and then use the external magnetic field to make the cells bound to the magnetic beads move in a directional manner to achieve the target cell separation. This method is simple and fast to operate, does not require large-scale instruments, and can obtain higher purity and recovery rate. When analyzing biological samples, it is often necessary to sort and mark bacteria. The publication number is the Chinese patent of CN 102243234 A , discloses a method for automatically sorting and marking bacteria based on an immune method, comprising the following steps: 1) magnetic beads are coated with antibody molecules; 2) fluorescent substances are coated with antibody molecules; 3) sample liquid is injected; 4) immunomagnetic beads 5) magnetic separation; 6) cleaning; 7) fluorescent substance marking target bacteria, the above-mentioned automatic bacterial sorting and marking method and device based on immune method cannot automatically quantitatively control antibody molecules according to the amount of sample liquid to be tested At the same time, the stirring function is not set, the working efficiency of the bacterial sorting marker is average, and the working progress of the bacterial sorting marker cannot be obtained in real time, so it is impossible to accurately grasp the working progress of the bacterial sorting marker

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