Sacha inchi small molecule peptide powder and preparation method thereof
A technology of small molecule peptides and saiteng fruit, which is applied in the field of satoba small molecule peptide powder and its preparation, can solve the problems of direct discarding of oil residue and environmental pollution, and achieve the effects of improving utilization rate, environmental friendliness and high efficiency
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Embodiment 1
[0028] A small molecular peptide powder of Mei Teng fruit, and its preparation method includes the following steps:
[0029] 1. Dry the Miteng fruit oil residue at 60°C for 30 minutes, and then crush the dried Miteng fruit oil residue and sieve 70 mesh;
[0030] 2. Add the sieved Miteng fruit oil residue powder to 2 times the volume of petroleum ether and soak for 48h to degreasing. After the degreasing treatment, the petroleum ether is recovered and the petroleum ether is volatilized clean to obtain the Miteng fruit oil residue defatted powder;
[0031] 3. Mix the defatted vine fruit powder with 2 times the volume of water to form a mixed solution, heat to 95°C, keep the temperature for 10 minutes, and cool to obtain the mixed solution;
[0032] 4. Add alkaline protease to the cooled mixed solution (the mass ratio of the mixed solution and alkaline protease is 22:1), adjust the pH to 8, heat to 50°C, and keep it for 3 hours to obtain the first enzymolysis solution ; Heat the enzymoly...
Embodiment 2
[0036] 1. Dry the Miteng fruit oil residue at a temperature of 50°C for 40 minutes, and then crush the dried Miteng fruit oil residue and sieve 80 mesh.
[0037] 2. The sieved M. Teng fruit oil residue powder is added to 3 times the volume of petroleum ether and soaked for 48 hours to defatted. After the degreasing treatment, the petroleum ether is recovered and the petroleum ether is volatilized clean to obtain M. Teng fruit oil residue defatted powder.
[0038] 3. Mix the defatted vine fruit powder with 3 times the volume of water to form a mixed solution, heat to 100°C, keep the temperature for 8 minutes, and cool to obtain the mixed solution.
[0039] 4. Add alkaline protease to the cooled mixed solution (the mass ratio of the mixed solution and alkaline protease is 25:1), adjust the pH to 10, heat to 55°C, and keep for 2 hours to obtain the first enzymolysis solution ; The enzymatic hydrolysate is heated to 85°C, kept for 10 minutes to inactivate the enzyme, and cooled to room t...
Embodiment 3
[0043] 1. Dry the Miteng fruit oil residue at 55°C for 35 minutes, then crush the dried Miteng fruit oil residue and sieve 80 mesh;
[0044] 2. Add the sieved M. Teng fruit oil residue powder to 5 times the volume of petroleum ether and soak for 48 hours to degreasing, and after the degreasing treatment, recover the petroleum ether and volatilize the petroleum ether to obtain M. Teng fruit oil residue defatted powder;
[0045] 3. Mix the defatted vine fruit powder with 2.5 times the volume of water into a mixed solution, heat to 90°C, keep it for 12 minutes, and cool to obtain the mixed solution;
[0046] 4. Add alkaline protease (the mass ratio of the mixed solution and the alkaline protease is 20:1) to the cooled mixed solution, adjust the pH to 9.0, heat to 50°C, and keep for 2.5 hours to obtain the first enzymatic hydrolysis solution ; Heat the enzymolysis solution to 85°C, keep it for 20min to inactivate the enzyme, and cool to room temperature;
[0047] 5. Add neutral protease, ...
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