Method for measuring content of paeoniflorin through high performance liquid chromatography
A high-performance liquid chromatography and paeoniflorin technology, which is applied in the field of high-performance liquid chromatography to determine the content of paeoniflorin, can solve the problems of few quality control methods for monoterpene glycosides, and achieve high sensitivity, good specificity, and cost-saving effects
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Embodiment 1
[0030] This embodiment relates to the detection method of paeoniflorin in Cortex Moutan, comprising the following steps:
[0031] A, prepare the standard curve of paeoniflorin content
[0032] 1) Precisely draw 5 μL, 10 μL, 20 μL, 25 μL and 50 μL of 1000 μg / mL paeoniflorin standard solution, place them in 10 mL colorimetric tubes, dilute to the mark with methanol, and shake well. Gradient standard solutions with concentrations of 0.5 μg / mL, 1 μg / mL, 2 μg / mL, 5 μg / mL and 10 μg / mL were obtained.
[0033] 2) Using a C18 chromatographic column, using phosphoric acid with a volume fraction of 0.02% as mobile phase A, and a mixture of methanol and acetonitrile as mobile phase B at a volume ratio of 5:1, gradient elution is performed, and the gradient elution program is 0 -3min mobile phase B volume fraction increased to 15%, 3-5min mobile phase B volume fraction increased from 15% to 30%, 5-10min mobile phase B volume fraction maintained at 30%, 10-15min, mobile phase The volume f...
experiment example 1
[0044] This experimental example involves the detection of the specificity of the method of the present invention, including the following steps: under the same conditions as in Example 1, the 2.0 μg / mL standard solution and the sample were measured respectively, and the concentration of paeoniflorin at 8.029min was compared. spectrum, see figure 2 . figure 2 It shows that the impurity in the sample and paeoniflorin have been well separated, and the retention time of paeoniflorin in the sample is exactly the same as that in the standard solution, and the method has good specificity.
experiment example 2
[0046] This experimental example relates to the mensuration of method precision of the present invention, comprises the steps:
[0047] 1) Instrument precision
[0048] Under the optimal conditions of the experiment, three standard solutions with a concentration of 2 μg / mL were measured in parallel, and the results are shown in Table 2.
[0049] Table 2 Precision (n=3)
[0050]
[0051] It can be seen from Table 2 that the relative standard deviation RSD is 0.58%, and the precision of the test method meets the requirements.
[0052] 2) Method precision
[0053] When using the method "3.1 Sample processing method" to process the sample, weigh three samples of 1.0000g respectively, and calculate the intraday precision. The measurement results are shown in Table 3.
[0054] Table 3 Intra-day precision
[0055]
[0056] It can be seen from Table 3 that the relative standard deviation RSD is 0.15%, and the method has good reproducibility.
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