A kind of method for producing rhamnolipid biosurfactant by solid-state fermentation

A solid-state fermentation, biological surface technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve problems such as inability to provide sufficient dissolved oxygen, achieve broad market application prospects, reduce production costs, and solve technical bottlenecks Effect

Active Publication Date: 2021-07-16
金元糖耕保新材料(西安)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of rhamnolipids by solid-state fermentation is usually not used by researchers. The reason is that the production of rhamnolipids by Pseudomonas aeruginosa is a strictly aerobic process, and general solid-state fermentation methods cannot provide sufficient dissolved oxygen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A method for producing rhamnolipid biosurfactant by solid-state fermentation is prepared by the following steps:

[0057] 1) Primary seed culture: Sterilize the liquid seed medium at 115°C for 20 minutes, inoculate Pseudomonas aeruginosa strains into the liquid seed medium, and cultivate on a shaking table to obtain the primary seed liquid;

[0058] 2) Secondary cultivation: put 8L liquid seed medium into a 15L mechanically stirred fermenter, sterilize at 115°C for 20 minutes, and cool to 30°C; inoculate the primary seed liquid into the cooled fermentation medium, and cultivate When the seed liquid grows to OD600=12, the cultivation is stopped, and the secondary seed liquid is obtained;

[0059] 3) Pressure pulsation solid-state fermentation: Fill the solid-state fermentation medium into a 50L pressure pulsation solid-state fermentation tank according to the filling coefficient of 0.50, sterilize at 125°C for 30 minutes, and cool to 30°C; inoculate the secondary seed li...

Embodiment 2

[0072] A method for producing rhamnolipid biosurfactant by solid-state fermentation is prepared by the following steps:

[0073] 1) Primary seed culture: Sterilize the liquid seed medium at 115°C for 30 minutes, inoculate Pseudomonas aeruginosa strains into the liquid seed medium, and cultivate on a shaking table to obtain the primary seed liquid;

[0074] 2) Secondary cultivation: put 8L liquid seed medium into a 15L mechanically stirred fermenter, sterilize at 115°C for 20 minutes, and cool to 30°C; inoculate the primary seed liquid into the cooled fermentation medium, and cultivate When the seed liquid grows to OD600=12, the cultivation is stopped, and the secondary seed liquid is obtained;

[0075] 3) Pressure pulsation solid-state fermentation: Fill the solid-state fermentation medium into a 50L pressure pulsation solid-state fermentation tank according to the filling factor of 0.4, sterilize at 125°C for 30 minutes, and cool to 30°C; inoculate the secondary seed liquid i...

Embodiment 3

[0088] A method for producing rhamnolipid biosurfactant by solid-state fermentation is prepared by the following steps:

[0089] 1) Primary seed culture: Sterilize the liquid seed medium at 115°C for 10 minutes, inoculate Pseudomonas aeruginosa strains into the liquid seed medium, and cultivate on a shaking table to obtain the primary seed liquid;

[0090] 2) Secondary cultivation: put 8L liquid seed medium into a 15L mechanically stirred fermenter, sterilize at 115°C for 20 minutes, and cool to 30°C; inoculate the primary seed liquid into the cooled fermentation medium, and cultivate When the seed liquid grows to OD600=12, the cultivation is stopped, and the secondary seed liquid is obtained;

[0091] 3) Pressure pulsation solid-state fermentation: Fill the solid-state fermentation medium into a 50L pressure pulsation solid-state fermentation tank according to the filling coefficient of 0.55, sterilize at 125°C for 30 minutes, and cool to 30°C; inoculate the secondary seed li...

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PUM

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Abstract

The invention discloses a method for producing rhamnolipid biosurfactant by solid-state fermentation, which is prepared by the following steps: first-level seed cultivation; second-level cultivation; pressure pulsation solid-state fermentation; and loading fermentation materials into filter bags , extruded to obtain a fermented liquid; oxalic acid to adjust the pH of the fermented liquid to 5.0, and the cut-off is 5Kda ultrafiltration membrane filtration to obtain a concentrated solution; oxalic acid to adjust the pH of the concentrated liquid to 3.0, centrifuge at 3500rpm to get the precipitate, and dry to obtain rhamnolipid biosurfactant. The invention has the following advantages: high output, less product impurities, low energy consumption, easy operation, simple post-treatment, less pollution, and uniform fermentation environment; polyurethane foam, an inert porous material, has stable properties, strong water holding capacity, can be reused, and significantly reduces production cost; in addition, no foam is generated during the entire fermentation process, which solves the technical bottleneck of severe foaming in the rhamnolipid production process and accelerates the industrialization process of rhamnolipid biosurfactants.

Description

technical field [0001] The invention belongs to the field of microbial fermentation technology and biosynthesis of natural products, and in particular relates to a method for producing rhamnolipid biosurfactant by solid-state fermentation. Background technique [0002] Surfactant is an important class of chemical raw materials, which occupies an important position in many fields such as petroleum industry, medicine, agriculture and fine chemical industry, and is known as "industrial monosodium glutamate". According to incomplete statistics, the production of surfactants in my country in 2016 was about 2 million tons, and almost all of them were petroleum-based surfactants. The resulting environmental problems have also become increasingly prominent. Compared with traditional chemically synthesized surfactants, biosurfactants represented by rhamnolipids have the advantages of being non-toxic, renewable, and easily biodegradable. At the same time, biodegradable surfactants ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/44C12R1/385
CPCC12P19/44
Inventor 巩志金杨革车程川刘金锋贺秋红
Owner 金元糖耕保新材料(西安)股份有限公司
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